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Test Code VITK1 Vitamin K1, Serum

Useful For

Assessment of circulating Vitamin K1 concentration

Method Name

Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)

Reporting Name

Vitamin K1, S

Specimen Type

Serum


Shipping Instructions


Ship specimen in amber vial or wrapped to protect from light.



Specimen Required


Patient Preparation:

Fasting: 12 hours, required; Infants should have specimen collected before next feeding

Supplies: Amber Frosted Tube, 5 mL (T915)

Collection Container/Tube:

Preferred: Red top

Acceptable: Serum gel

Submission Container/Tube: Amber vial

Specimen Volume: 2 mL Serum

Collection Instructions:

1. Within 2 hours of collection, centrifuge the specimen.

2. For red-top tubes:
Preferred: Immediately aliquot serum into an amber or light-protected plastic vial.

Acceptable: Serum may be on whole blood cells a maximum of 2 hours before aliquoting. Protect the collection tube from light.

3. For serum-gel tubes:

Preferred: Immediately aliquot serum into an amber or light-protected plastic vial.

Acceptable: Serum may be aliquoted into an amber or light-protected plastic vial within 24 hours of collection when kept at ambient temperature and protected from light or within 7 days of collection if kept refrigerated and protected from light.


Specimen Minimum Volume

Serum: 0.75 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 14 days
  Frozen  30 days
  Ambient  24 hours

Reference Values

<18 years: Not established

≥18 years: 0.10-2.20 ng/mL

Interpretation

Low vitamin K1 concentrations in the serum are indicative of insufficiency and poor vitamin K1 status.

Clinical Reference

1. Vitamin K-Fact Sheet for Health Professionals. US Department of Health and Human Services, National Institutes of Health. Office of Dietary Supplements. Updated March 29, 2021. Accessed April 17, 2026. Available at: https://ods.od.nih.gov/factsheets/VitaminK-HealthProfessional/

2. Zhang Y, Bala V, Mao Z, Chhonker YS, Murry DJ. A concise review of quantification methods for determination of vitamin K in various biological matrices. J Pharm Biomed Anal. 2019;169:133-141. doi:10.1016/j.jpba.2019.03.006

3. Sodi R, Taylor A. Vitamins and trace elements In: Rifai N, Horvath AR, Wittwer CT, eds. Tietz Fundamentals of Clinical Chemistry and Molecular Diagnostics. 8th ed. Elsevier; 2020:466-487

4. Booth SL. Vitamin K: food composition and dietary intakes. Food Nutr Res. 2012;56 :10.3402/fnr.v56i0.5505. doi:10.3402/fnr.v56i0.5505

5. Shearer MJ, Newman P. Metabolism and cell biology of vitamin K. Thromb Haemost. 2008;100(4):530-547

6. Mladenka P, Macakova K, Kujovska Krcmova L, et al. Vitamin K - sources, physiological role, kinetics, deficiency, detection, therapeutic use, and toxicity. Nutr Rev. 2022;80(4):677-698. doi:10.1093/nutrit/nuab061

7. Card DJ, Gorska R, Harrington DJ. Laboratory assessment of vitamin K status. J Clin Pathol. 2020;73(2):70-75. doi:10.1136/jclinpath-2019-205997

Day(s) Performed

Monday through Friday

Report Available

2 to 5 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

84597

LOINC Code Information

Test ID Test Order Name Order LOINC Value
VITK1 Vitamin K1, S 9622-2

 

Result ID Test Result Name Result LOINC Value
62167 Vitamin K1, S 9622-2

Forms

If not ordering electronically, complete, print, and send a General Request (T239) with the specimen.