Sign in →

Test Code VITK1 Vitamin K1, Serum

Useful For

Assessment of circulating vitamin K1 concentration.

Method Name

Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)

Reporting Name

Vitamin K1, S

Specimen Type

Serum


Specimen Required


Patient Preparation: Patient should fast overnight (12-14 hours); infants should have specimen collected before next feeding.

Supplies: Amber Frosted Tube, 5 mL (T915)

Collection Container/Tube:

Preferred: Red top

Acceptable: Serum gel

Submission Container/Tube: Amber vial

Specimen Volume: 2 mL

Collection Instructions:

1. Within 2 hours of collection, centrifuge the specimen.

2. For red top tubes, immediately aliquot serum into an amber vial (preferred). Serum may sit on whole blood cells up to a maximum of two hours before aliquoting.

3. For serum gel tubes, immediately aliquot serum into an amber vial (preferred). Serum may sit on gel at ambient temperature for a maximum of 24 hours or refrigerated for a maximum of 7 days before aliquoting.


Specimen Minimum Volume

0.75 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 30 days
  Ambient  30 days
  Frozen  30 days

Reference Values

<18 years: Not established

≥18 years: 0.10-2.20 ng/mL

Interpretation

Low vitamin K1 concentrations in the serum are indicative of insufficiency and poor vitamin K1 status.

Clinical Reference

1. Vitamin K-Fact Sheet for Health Professionals. US Department of Health and Human Services, National Institutes of Health. Office of Dietary Supplements. Updated March 29, 2021. Accessed April 9, 2025. Available at: https://ods.od.nih.gov/factsheets/VitaminK-HealthProfessional/

2. Zhang Y, Bala V, Mao Z, Chhonker YS, Murry DJ. A concise review of quantification methods for determination of vitamin K in various biological matrices. J Pharm Biomed Anal. 2019;169:133-141. doi:10.1016/j.jpba.2019.03.006

3. Sodi R, Taylor A. Vitamins and trace elements In: Rifai N, Horvath AR, Wittwer CT, eds. Tietz Fundamentals of Clinical Chemistry and Molecular Diagnostics. 8th ed. Elsevier; 2020:466-487

4. Booth SL. Vitamin K: food composition and dietary intakes. Food Nutr Res. 2012;56 :10.3402/fnr.v56i0.5505. doi:10.3402/fnr.v56i0.5505

5. Shearer MJ, Newman P. Metabolism and cell biology of vitamin K. Thromb Haemost. 2008;100(4):530-547

6. Mladenka P, Macakova K, Kujovska Krcmova L, et al. Vitamin K - sources, physiological role, kinetics, deficiency, detection, therapeutic use, and toxicity. Nutr Rev. 2022;80(4):677-698. doi:10.1093/nutrit/nuab061

7. Card DJ, Gorska R, Harrington DJ. Laboratory assessment of vitamin K status. J Clin Pathol. 2020;73(2):70-75. doi:10.1136/jclinpath-2019-205997

Day(s) Performed

Monday through Friday

Report Available

2 to 5 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

84597

LOINC Code Information

Test ID Test Order Name Order LOINC Value
VITK1 Vitamin K1, S 9622-2

 

Result ID Test Result Name Result LOINC Value
62167 Vitamin K1, S 9622-2

Forms

If not ordering electronically, complete, print, and send a General Request (T239) with the specimen.