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Test Code VITB2 Riboflavin (Vitamin B2), Plasma

Important Note

  • Must collect Large Green Sodium (Na) Heparin/No Gel
  • Split IMMEDIATELY
  • Aliquote in Amber Vial
  • Refrigerate after aliquoting
  • PROTECT FROM LIGHT

Useful For

Assessment of riboflavin (vitamin B2) status

 

Assisting in the diagnosis of suspected vitamin B2 deficiency

 

Monitoring of vitamin B2 therapy

Method Name

Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)

 

Portions of this test are covered by patents held by Quest Diagnostics

Reporting Name

Riboflavin (Vitamin B2), P

Specimen Type

Plasma Heparin


Shipping Instructions


Ship specimen in amber vial to protect from light.



Specimen Required


Patient Preparation:

Fasting: 12 hours, required; Infants should have specimen collected before next feeding

Supplies: Amber Frosted Tube, 5 mL (T915)

Collection Container/Tube:

Preferred: Green top (sodium or lithium heparin)

Acceptable: Light-green top (sodium or lithium heparin plasma gel)

Submission Container/Tube: Amber vial

Specimen Volume: 2 mL Plasma

Collection Instructions: Within 2 hours of collection, centrifuge and aliquot plasma into a amber vial.


Specimen Minimum Volume

Plasma: 0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Plasma Heparin Refrigerated (preferred) 28 days LIGHT PROTECTED
  Frozen  28 days LIGHT PROTECTED
  Ambient  72 hours LIGHT PROTECTED

Reference Values

1-19 mcg/L

Interpretation

Low concentrations of vitamin B2 in the plasma are indicative of nutritional deficiency. Concentrations below 1 mcg/L are indicative of deficiency.

Clinical Reference

1. Sodi R, Taylor A. Vitamins and trace elements. In: Rifai N, Horvath AR, Wittwer CT, eds. Tietz Fundamentals of Clinical Chemistry and Molecular Diagnostics. 8th ed. Elsevier; 2020:466-487

2. Riboflavin- Fact Sheet for Health Professionals. US Department of Health and Human Services, National Institutes of Health. Updated May 11, 2022. Accessed March 17, 2026. Available at https://ods.od.nih.gov/factsheets/Riboflavin-HealthProfessional/

3. Hustad S, McKinley MC, McNulty H, et al. Riboflavin, flavin mononucleotide, and flavin adenine dinucleotide in human plasma and erythrocytes at baseline and after low-dose riboflavin supplementation. Clin Chem. 2002;48(9):1571-1577

4. Balasubramaniam S, Christodoulou J, Rahman S. Disorders of riboflavin metabolism. J Inherit Metab Dis. 2019;42(4):608-619. doi:10.1002/jimd.12058

5. Suwannasom N, Kao I, Pruss A, Georgieva R, Baumler H. Riboflavin: the health benefits of a forgotten natural vitamin. Int J Mol Sci. 2020;21(3):950. doi:10.3390/ijms21030950

6. O'Callaghan B, Bosch AM, Houlden H. An update on the genetics, clinical presentation, and pathomechanisms of human riboflavin transporter deficiency. J Inherit Metab Dis. 2019;42(4):598-607. doi:10.1002/jimd.12053

Day(s) Performed

Monday, Wednesday, Friday

Report Available

2 to 5 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

84252

LOINC Code Information

Test ID Test Order Name Order LOINC Value
VITB2 Riboflavin (Vitamin B2), P 2924-9

 

Result ID Test Result Name Result LOINC Value
61637 Riboflavin (Vitamin B2), P 2924-9