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HelpTest Code VASC Antineutrophil Cytoplasmic Antibodies Vasculitis Panel, Serum
Reporting Name
ANCA Panel for Vasculitis, SUseful For
Evaluating patients with clinical features of anti-neutrophil cytoplasmic antibody-associated vasculitis, specifically granulomatosis with polyangiitis, microscopic polyangiitis, and eosinophilic granulomatosis with polyangiitis
Profile Information
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| MPO | Myeloperoxidase Ab, S | Yes | Yes |
| PR3 | Proteinase 3 Ab (PR3), S | Yes | Yes |
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| ANCA | Cytoplasmic Neutrophilic Ab, S | Yes | No |
Testing Algorithm
If either the myeloperoxidase antibody or proteinase 3 antibody result is greater than or equal to 0.4 U, then cytoplasmic neutrophilic antibody testing will be performed at an additional charge.
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
SerumOrdering Guidance
For monitoring disease activity, order either PR3 / Proteinase 3 Antibodies, IgG, Serum or MPO / Myeloperoxidase Antibodies, IgG, Serum.
Specimen Required
Supplies: Sarstedt Aliquot Tube 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Specimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 21 days | |
| Frozen | 21 days |
Reference Values
MYELOPEROXIDASE ANTIBODIES, IgG
<0.4 U (negative)
0.4-0.9 U (equivocal)
≥1.0 U (positive)
Reference values apply to all ages.
PROTEINASE 3 ANTIBODIES, IgG
<0.4 U (negative)
0.4-0.9 U (equivocal)
≥1.0 U (positive)
Reference values apply to all ages.
Day(s) Performed
Monday through Saturday
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
83516 x 2
86036 x 2 (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| VASC | ANCA Panel for Vasculitis, S | 90230-4 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| MPO | Myeloperoxidase Ab, S | 48404-8 |
| PR3 | Proteinase 3 Ab (PR3), S | 74106-6 |
Interpretation
Positive results for proteinase 3 anti-neutrophil cytoplasmic antibodies (ANCA) by antigen-specific immunoassay and cytoplasmic ANCA by indirect immunofluorescence are consistent with the diagnosis of granulomatosis with polyangiitis, in patients with the appropriate clinical presentation.
Positive results for myeloperoxidase-ANCA by antigen-specific immunoassay and perinuclear ANCA by indirect immunofluorescence are consistent with the diagnosis of microscopic polyangiitis or eosinophilic granulomatosis with polyangiitis, in patients with the appropriate clinical presentation.
Clinical Reference
1. Kitching AR, Anders HJ, Basu N, et al. ANCA-associated vasculitis. Nat Rev Dis Primers. 2020;6(1):71
2. Ramponi G, Folci M, De Santis M, et al. The biology, pathogenetic role, clinical implications, and open issues of serum anti-neutrophil cytoplasmic antibodies. Autoimmun Rev. 2021;20(3):102759
3. Bossuyt X, Cohen Tervaert JW, Arimura Y, et al. Position paper: Revised 2017 international consensus on testing of ANCAs in granulomatosis with polyangiitis and microscopic polyangiitis. Nat Rev Rheumatol. 2017;13(11):683-692
4. Suppiah R, Robson JC, Grayson PC, et al. 2022 American College of Rheumatology/European Alliance of Associations for Rheumatology classification criteria for microscopic polyangiitis. Ann Rheum Dis. 2022;81(3):321-326. doi:10.1136/annrheumdis-2021-221796
5. Robson JC, Grayson PC, Ponte C, et al. 2022 American College of Rheumatology/European Alliance of Associations for Rheumatology classification criteria for granulomatosis with polyangiitis. Ann Rheum Dis. 2022;81(3):315-320. doi:10.1136/annrheumdis-2021-221795
6. Grayson PC, Ponte C, Suppiah R, et al. 2022 American College of Rheumatology/European Alliance of Associations for Rheumatology classification criteria for eosinophilic granulomatosis with polyangiitis. Ann Rheum Dis. 2022;81(3):309-314. doi:10.1136/annrheumdis-2021-221794
Report Available
3 to 4 daysMethod Name
Multiplex Flow Immunoassay
Forms
If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:
-General Request (T239)
-Renal Diagnostics Test Request (T830)