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HelpTest Code URPRP Ureaplasma species, Molecular Detection, PCR, Plasma
Useful For
Rapid, sensitive, and specific identification of Ureaplasma urealyticum and Ureaplasma parvum from plasma
This test is not intended for medicolegal use.
Method Name
Real-Time Polymerase Chain Reaction (PCR)
Reporting Name
Ureaplasma PCR, PSpecimen Type
Plasma EDTASpecimen Required
The high sensitivity of amplification by polymerase chain reaction requires the specimen to be processed in an environment in which contamination of the specimen by Ureaplasma DNA is unlikely.
Collection Container/Tube:
Preferred: Lavender top (EDTA)
Acceptable: Royal blue top (EDTA), pink top (EDTA), or sterile vial containing EDTA-derived aliquot
Submission Container/Tube: Screw-capped, sterile container
Specimen Volume: 1 mL Plasma
Collection Instructions: Centrifuge and aliquot plasma into a plastic vial.
Specimen Minimum Volume
Plasma: 0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Plasma EDTA | Refrigerated (preferred) | 7 days |
| Frozen | 7 days |
Reference Values
Not applicable
Interpretation
A positive polymerase chain reaction (PCR) result for the presence of a specific sequence found within the Ureaplasma urealyticum and Ureaplasma parvum ureC gene indicates the presence of U urealyticum or U parvum DNA in the specimen.
A negative PCR result indicates the absence of detectable U urealyticum and U parvum DNA in the specimen but does not rule out infection, as false-negative results may occur due to inhibition of PCR, sequence variability underlying the primers and probes, or the presence of U urealyticum or U parvum in quantities below the limit of detection of the assay.
Clinical Reference
1. Bharat A, Cunningham SA, Scott Budinger GR, et al. Disseminated Ureaplasma infection as a cause of fatal hyperammonemia in humans. Sci Transl Med. 2015;7(284):284re3
2. Wang X, Greenwood-Quaintance KE, Karau MJ, et al. Ureaplasma parvum causes hyperammonemia in a pharmacologically immunocompromised murine model. Eur J Clin Microbiol Infect Dis. 2017;36(3):517-522. doi:10.1007/s10096-016-2827-1
3. Fernandez J, Karau MJ, Cunningham SA, Greenwood-Quaintance KE, Patel R. Antimicrobial susceptibility and clonality of clinical Ureaplasma isolates in the United States. Antimicrob Agents Chemother. 2016;60(8):4793-4798
4. Al-Jabri MY, Patel R, Fleming D. Prior immunity to Ureaplasma urealyticum protects against respiratory infection in immunosuppressed mice. Microbiol Spectr. 2025;13(1):e0176324
Day(s) Performed
Monday through Sunday
Report Available
3 to 4 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
87798 x 2
87999 (if appropriate for government payers)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| URPRP | Ureaplasma PCR, P | 69934-8 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| UPSRC | Specimen Source | 31208-2 |
| 44135 | Ureaplasma urealyticum PCR, P | 51988-4 |
| 44136 | Ureaplasma parvum PCR, P | 69933-0 |