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HelpTest Code URPRP Ureaplasma species, Molecular Detection, PCR, Plasma
Useful For
Rapid, sensitive, and specific identification of Ureaplasma urealyticum and Ureaplasma parvum from plasma
This test is not intended for medicolegal use.
Method Name
Real-Time Polymerase Chain Reaction (PCR) using LightCycler and Fluorescent Resonance Energy Transfer (FRET)
Reporting Name
Ureaplasma PCR, PSpecimen Type
Plasma EDTASpecimen Required
The high sensitivity of amplification by polymerase chain reaction requires the specimen to be processed in an environment in which contamination of the specimen by Ureaplasma DNA is unlikely.
Collection Container/Tube:
Preferred: Lavender top (EDTA)
Acceptable: Royal blue top (EDTA), pink top (EDTA), or sterile vial containing EDTA-derived aliquot
Submission Container/Tube: Screw-capped, sterile container
Specimen Volume: 1 mL
Collection Instructions: Centrifuge and aliquot plasma into a plastic vial.
Specimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Plasma EDTA | Refrigerated (preferred) | 7 days | |
| Frozen | 7 days |
Reference Values
Not applicable
Interpretation
A positive polymerase chain reaction (PCR) result for the presence of a specific sequence found within the Ureaplasma urealyticum and Ureaplasma parvum ureC gene indicates the presence of U urealyticum or U parvum DNA in the specimen.
A negative PCR result indicates the absence of detectable U urealyticum and U parvum DNA in the specimen but does not rule out infection as false-negative results may occur due to inhibition of PCR, sequence variability underlying the primers and probes, or the presence of U urealyticum or U parvum in quantities below the limit of detection of the assay.
Clinical Reference
1. Bharat A, Cunningham SA, Scott Budinger GR, Kreisel D, et al: Disseminated Ureaplasma infection as a cause of fatal hyperammonemia in humans. Sci Transl Med. 2015;7(284):284re3
2. Stellrecht KA, Woron AM, Mishrik NG, Venezia RA: Comparison of multiplex PCR assay with culture detection of genital mycoplasmas. J Clin Microbiol. 2004 Apr;42(4):1528-1533
3. Farrell JJ, Larson JA, Akeson JW, et al: Ureaplasma parvum prosthetic joint infection detected by PCR. J Clin Microbiol. 2014;52:2248-2250
4. Waites KB, Taylor-Robinson D: Mycoplasma and Ureaplasma. In: Jorgensen JH, ed. Manual of Clinical Microbiology. 11th ed. ASM Press; 2015:1088-1105
5. Kenny GE: Genital mycoplasmas: Mycoplasma genitalium, Mycoplasma hominis, and Ureaplasma species. In: Mandell GL ed. Mandell, Douglas, and Bennett's Principles and Practice of Infectious Diseases. Churchill Livingstone; 2008
Day(s) Performed
Monday through Friday
Report Available
3 to 4 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
87798 x 2
87999 (if appropriate for government payers)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| URPRP | Ureaplasma PCR, P | 69934-8 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| UPSRC | Specimen Source | 31208-2 |
| 44135 | Ureaplasma urealyticum PCR, P | 51988-4 |
| 44136 | Ureaplasma parvum PCR, P | 69933-0 |