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Test Code URBRP Ureaplasma species, Molecular Detection, PCR, Blood

Useful For

Rapid, sensitive, and specific identification of Ureaplasma urealyticum and Ureaplasma parvum from whole blood

 

This test is not intended for medicolegal use.

Method Name

Real-Time Polymerase Chain Reaction (PCR) using LightCycler and Fluorescent Resonance Energy Transfer (FRET)

Reporting Name

Ureaplasma PCR, B

Specimen Type

Whole Blood EDTA


Specimen Required


The high sensitivity of amplification by polymerase chain reaction requires the specimen to be processed in an environment in which contamination of the specimen by Ureaplasma DNA is not likely.

 

Container/Tube:

Preferred Lavender top (EDTA)

Acceptable: Royal blue top (EDTA), pink top (EDTA), or sterile vial containing EDTA-derived aliquot

Specimen Volume: 1 mL

Collection Instructions: Send whole blood specimen in original tube (preferred


Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Whole Blood EDTA Refrigerated (preferred) 7 days
  Frozen  7 days

Reference Values

Not applicable

Interpretation

A positive PCR result for the presence of a specific sequence found within the Ureaplasma urealyticum and U parvum ureC gene indicates the presence of U urealyticum or U parvum DNA in the specimen.

 

A negative PCR result indicates the absence of detectable U urealyticum and U parvum DNA in the specimen, but does not rule-out infection as false-negative results may occur due to inhibition of PCR, sequence variability underlying the primers and probes, or the presence of U urealyticum or U parvum in quantities less than the limit of detection of the assay.

Clinical Reference

1. Bharat A, Cunningham SA, Scott Budinger GR, Kreisel D, et al: Disseminated Ureaplasma infection as a cause of fatal hyperammonemia in humans. Sci Transl Med 2015;7(284):284re3

2. Stellrecht KA, Woron AM, Mishrik NG, Venezia RA: Comparison of multiplex PCR assay with culture detection of genital mycoplasmas. J Clin Microbiol 2004;42:1528-1533

3. Farrell JJ, Larson JA, Akeson JW, et al: Ureaplasma parvum prosthetic joint infection detected by PCR. J Clin Microbiol 2014;52:2248-2250

4. Waites KB, Taylor-Robinson D: Mycoplasma and Ureaplasma. In: Jorgensen JH, ed. Manual of Clinical Microbiology. 11th ed. ASM Press; 2015:1088-1105

5. Kenny GE: Genital mycoplasmas: Mycoplasma genitalium, Mycoplasma hominis, and Ureaplasma species. In: Bennett JE, Dolin R, Blaser MJ, eds. Mandell, Douglas, and Bennett's Principles and Practice of Infectious Diseases. Churchill Livingstone;2020:chap 184

Day(s) Performed

Monday through Friday

Report Available

3 to 4 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

87798 x 2

87999 (if appropriate for government payers)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
URBRP Ureaplasma PCR, B 69934-8

 

Result ID Test Result Name Result LOINC Value
UBSRC Specimen Source 31208-2
44132 Ureaplasma urealyticum PCR, B 51988-4
44133 Ureaplasma parvum PCR, B 69933-0