Home
HelpTest Code TRYPT Tryptase, Serum
Reporting Name
Tryptase, SUseful For
Evaluation of individuals with suspected mast cell activation, which may occur as a result of anaphylaxis or allergen challenge
Evaluation of patients with suspected cutaneous or systemic mastocytosis
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
SerumSpecimen Required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions: Centrifuge and aliquot serum into plastic vial
Additional Information: Tryptase degenerates very quickly when left in the presence of red blood cells.
Specimen Minimum Volume
0.2 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Frozen (preferred) | 14 days | |
| Refrigerated | 7 days |
Reference Values
<11.5 ng/mL
Day(s) Performed
Monday through Friday
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
83520
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| TRYPT | Tryptase, S | 21582-2 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| TRYPT | Tryptase, S | 21582-2 |
Interpretation
Transient tryptase concentrations greater than or equal to 11.5 ng/mL may be consistent with mast cell activation in the context of anaphylaxis or allergen challenge; measurement of tryptase in specimens obtained 1 to 6 hours and at least 24 hours after the episode may be useful in demonstrating a return to baseline concentrations.
Basal tryptase concentrations greater than or equal to 11.5 mg/mL may be consistent with cutaneous mastocytosis.
Basal tryptase concentrations greater than or equal to 20 ng/mL may be consistent with systemic mastocytosis.
Clinical Reference
1. Lyons JJ, Yi T: Mast cell tryptases in allergic inflammation and immediate hypersensitivity. Curr Opin Immunol. 2021 Oct;72:94-106. doi: 10.10.16/j.coi.2021.04.001
2. Platzgummer S, Bizzaro N, Bilo MB, et al: Recommendations for the use of tryptase in the diagnosis of anaphylaxis and clonal mastcell disorders. Eur Ann Allergy Clin Immunol. 2020 Mar;52(2):51-61. doi: 10.23822/EurAnnACI.1764-1489.133
3. Theoharides TC, Valent P, Akin C: Mast cells, mastocytosis, and related disorders. N Engl J Med. 2015 Jul 9;373(2):163-172. doi: 10.1056/NEJMra1409760
Report Available
2 to 5 daysMethod Name
Fluorescence Enzyme Immunoassay (FEIA)
Forms
If not ordering electronically, complete, print, and send a General Request (T239) with the specimen.
Testing Algorithm
For more information, see Mast Cell Disorder: Diagnostic Algorithm, Bone Marrow