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HelpTest Code TRYPT Tryptase, Serum
Reporting Name
Tryptase, SUseful For
Evaluation of individuals with suspected mast cell activation, which may occur as a result of anaphylaxis or allergen challenge
Evaluation of patients with suspected mast cell activation syndrome
Evaluation of patients with suspected cutaneous or systemic mastocytosis
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
SerumSpecimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial
Additional Information: Tryptase degenerates very quickly when left in the presence of red blood cells.
Specimen Minimum Volume
0.2 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Frozen (preferred) | 14 days |
| Refrigerated | 7 days |
Reference Values
<11.5 ng/mL
Day(s) Performed
Monday through Friday
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
83520
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| TRYPT | Tryptase, S | 21582-2 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| TRYPT | Tryptase, S | 21582-2 |
Interpretation
Transient tryptase concentrations greater than or equal to 11.5 ng/mL may be consistent with mast cell activation in the context of anaphylaxis or allergen challenge; measurement of tryptase in specimens obtained 1 to 6 hours and at least 24 hours after the episode may be useful in demonstrating a return to baseline concentrations.
Basal tryptase concentrations greater than or equal to 11.5 mg/mL may be consistent with cutaneous mastocytosis.
Basal tryptase concentrations greater than or equal to 20 ng/mL may be consistent with systemic mastocytosis.
Clinical Reference
1. Lyons JJ, Yi T. Mast cell tryptases in allergic inflammation and immediate hypersensitivity. Curr Opin Immunol. 2021;72:94-106. doi:10.1016/j.coi.2021.04.001
2. Lyons JJ, Sun G, Stone KD, et al. Mendelian inheritance of elevated serum tryptase associated with atopy and connective tissue abnormalities. J Allergy Clin Immunol. 2014;133(5):1471-1474. doi:10.1016/j.jaci.2013.11.039
3. Platzgummer S, Bizzaro N, Bilo MB, et al. Recommendations for the use of tryptase in the diagnosis of anaphylaxis and clonal mastcell disorders. Eur Ann Allergy Clin Immunol. 2020;52(2):51-61. doi:10.23822/EurAnnACI.1764-1489.133
4. Valent P, Akin C, Bonadonna P, et al. Proposed diagnostic algorithm for patients with suspected mast cell activation syndrome. J Allergy Clin Immunol Pract. 2019;7(4):1125-1133.e1. doi:10.1016/j.jaip.2019.01.006
5. Valent P, Akin C, Hartmann K, et al. Updated diagnostic criteria and classification of mast cell disorders: A consensus proposal. Hemasphere. 2021;5(11):e646. doi:10.1097/HS9.0000000000000646
6. Valent P, Hoermann G, Bonadonna P, et al. The normal range of baseline tryptase should be 1 to 15 ng/mL and covers healthy individuals with HalphaT. J Allergy Clin Immunol Pract. 2023;11(10):3010-3020. doi:10.1016/j.jaip.2023.08.008
Report Available
2 to 5 daysMethod Name
Fluorescence Enzyme Immunoassay (FEIA)
Forms
If not ordering electronically, complete, print, and send a General Request (T239) with the specimen.
Testing Algorithm
For more information see Mast Cell Disorder: Diagnostic Algorithm, Bone Marrow.