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HelpTest Code TRYPA Tryptase, Autopsy, Serum
Reporting Name
Tryptase, AutopsyUseful For
Evaluation of autopsy cases in which anaphylaxis in the context of allergen exposure or mast cell activation is a suspected cause of death
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
SerumSpecimen Required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions: Centrifuge and aliquot serum into plastic vial.
Additional Information: Tryptase degenerates very quickly when left in the presence of red blood cells.
Specimen Minimum Volume
0.2 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Frozen (preferred) | 14 days | |
| Refrigerated | 7 days |
Reference Values
No established reference values
Day(s) Performed
Monday through Friday
Test Classification
This test has been modified from the manufacturer's instructions. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
83520
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| TRYPA | Tryptase, Autopsy | 21582-2 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| TRYPA | Tryptase, Autopsy | 21582-2 |
Interpretation
Increased concentrations of total tryptase may indicate mast cell activation occurring as a result of anaphylaxis or allergen challenge, or it may indicate an increased number of mast cells as seen in patients with mastocytosis. However, no specific cutoff value has been widely validated for autopsy specimens.
Clinical Reference
1. Lyons JJ, Yi T: Mast cell tryptases in allergic inflammation and immediate hypersensitivity. Curr Opin Immunol. 2021;72:94-106. doi: 10.10.16/j.coi.2021.04.001
2. Platzgummer S, Bizzaro N, Bilo MB, et al: Recommendations for the use of tryptase in the diagnosis of anaphylaxis and clonal mast cell disorders. Eur Ann Allergy Clin Immunol. 2020 Mar;52(2):51-61. doi:10.23822/EurAnnACI.1764-1489.133
3. Garland J, Ondruschka B, Broi UD, et al: Post mortem tryptase: a review of literature on its use, sampling and interpretation in the investigation of fatal anaphylaxis. Forensic Sci Int. 2020 Sep;314:110415. doi: 10.1016/j.forsciint.2020.110415
4. Tse R, Wong CX, Kesha K, et al: Post mortem tryptase cut-off level for anaphylactic death. Foresnic Sci Int. 2018 Mar;284:5-8. doi: 10.1016/j.forsciint.2017.12.035
Report Available
2 to 5 daysMethod Name
Fluorescence Enzyme Immunoassay (FEIA)