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HelpTest Code TRBC1 T-Cell Receptor Constant Beta Chain (TRBC1) Immunostain, Technical Component Only
Ordering Guidance
This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.
Shipping Instructions
Attach the green "Attention Pathology" address label (T498) and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.
Specimen Required
Specimen Type: Tissue
Supplies: Immunostain Technical Only Envelope (T693)
Container/Tube: Immunostain Technical Only Envelope
Submit:
-Formalin-fixed, paraffin-embedded tissue block
OR
-2 Unstained, positively charged glass slides (25- x 75- x 1-mm) per test ordered; sections 4-microns thick
Useful For
Determination of T-cell clonality in T-cell neoplasms
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| IHTOI | IHC Initial, Tech Only | No | No |
| IHTOA | IHC Additional, Tech Only | No | No |
Testing Algorithm
For the initial technical component only immunohistochemical (IHC) stain performed, the appropriate bill-only test ID will be reflexed and charged (IHTOI). For each additional technical component only IHC stain performed, an additional bill-only test ID will be reflexed and charged (IHTOA).
Method Name
Immunohistochemistry (IHC)
Reporting Name
TRBC1 IHC, Tech OnlySpecimen Type
TECHONLYSpecimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| TECHONLY | Ambient (preferred) | |
| Refrigerated | ||
Interpretation
This test does not include pathologist interpretation, only technical performance of the stain. If interpretation is required, order PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case.
The positive and negative controls are verified as showing appropriate immunoreactivity. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request; call 855-516-8404.
Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Clinical Reference
1. Zhou T, Sardana R, Eren OC, et al. The diagnostic utility of TRBC1 immunohistochemistry in mature T-cell lymphomas. Mod Pathol. Published online January 28,2025.. doi:10.1016/j.modpat.2025.100725
2. Horna P, Weybright MJ, Ferrari M, et al. Dual T-cell constant beta chain (TRBC)1 and TRBC2 staining for the identification of T-cell neoplasms by flow cytometry. Blood Cancer J. 2024;14(1):34
3. Soilleux EJ, Rodgers DT, Situ JJ, et al. Demonstration of T-cell monotypia using anti-TCRbeta1/2 (TRBC1/2) immunostaining as a rapid and cost-effective alternative to PCR-based clonality studies for the diagnosis of T-cell lymphoma. Diagnostics (Basel). 2024;14(22):2479
4. Nocco SE, Ewalt MD, Moy AP, et al. TRBC1 immunohistochemistry distinguishes cutaneous T-cell lymphoma from inflammatory dermatitis: A retrospective analysis of 39 cases. J Am Acad Dermatol. 2024;90(4):839-841
Day(s) Performed
Monday through Friday
Report Available
1 to 3 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
88342-TC, Primary
88341-TC, If additional IHC
LOINC Code Information
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 622853 | TRBC1 IHC, Tech Only | No LOINC Needed |