Test Code TOXB Toxoplasma gondii, Molecular Detection, PCR, Blood
Useful For
Supporting the diagnosis of active toxoplasmosis, particularly in immunocompromised individuals
Reporting Name
Toxoplasma gondii PCR, BSpecimen Type
Whole Blood EDTASpecimen Required
Container/Tube: Lavender top (EDTA)
Specimen Volume: 1 mL
Collection Instructions: Send whole blood specimen in original tube. Do not aliquot.
Specimen Minimum Volume
0.3 mL
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Whole Blood EDTA | Refrigerated (preferred) | 7 days | |
Frozen | 7 days |
Reference Values
Negative
Interpretation
A positive result indicates presence of DNA from Toxoplasma gondii.
Negative results indicate absence of detectable DNA, but do not exclude the presence of organism or active or recent disease.
Clinical Reference
1. Robert-Gangneux F, Darde M. Epidemiology of and diagnostic strategies for toxoplasmosis. Clin Microbiol Rev. 2012 Apr ;25(2):264-296
2. McAuley JB, Singh K: Toxoplasma, In: Carroll KC, Pfaller MA, eds. Manual of Clinical Microbiology, 12th ed. ASM Press; 2019: chap141.
3. Mattos CCB, Meira CS, Ferreira AIC, et al: Contribution of laboratory methods in diagnosing clinically suspected ocular toxoplasmosis in Brazilian patients. Diagn Microbiol Infec Dis. 2011 Jul;70(3):362-366
4. Martino R, Bretagne S, Einsele H, et al: Early detection of Toxoplasma infection by molecular monitoring of Toxoplasma gondii in peripheral blood samples after allogeneic stem cell transplantation. Clin Infect Dis. 2005 Jan 1;40(1):67-78
5. Fricker-Hidalgo H, Bulabois C, Brenier-Pinchart M, et al: Diagnosis of toxoplasmosis after allogeneic stem cell transplantation: results of DNA detection and serological techniques. Clin Infect Dis. 2009;48:e9-e15
Day(s) Performed
Monday through Saturday
Report Available
Same day/1 to 4 daysPerforming Laboratory
Mayo Clinic Laboratories in RochesterTest Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
87798
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
TOXB | Toxoplasma gondii PCR, B | 16279-2 |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
62977 | Toxoplasma gondii PCR, B | 16279-2 |
Method Name
Polymerase Chain Reaction (PCR)/DNA Probe Hybridization
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.