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HelpTest Code TOXB Toxoplasma gondii, Molecular Detection, PCR, Blood
Useful For
Supporting the diagnosis of active toxoplasmosis, particularly in immunocompromised individuals
Reporting Name
Toxoplasma gondii PCR, BSpecimen Type
Whole Blood EDTASpecimen Required
Container/Tube: Lavender top (EDTA)
Specimen Volume: 1 mL
Collection Instructions: Send whole blood specimen in original tube. Do not aliquot.
Specimen Minimum Volume
0.3 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Whole Blood EDTA | Refrigerated (preferred) | 7 days |
| Frozen | 7 days |
Reference Values
Negative
Interpretation
A positive result indicates presence of DNA from Toxoplasma gondii.
Negative results indicate absence of detectable DNA, but do not exclude the presence of organism or active or recent disease.
Clinical Reference
1. Robert-Gangneux F, Darde ML. Epidemiology of and diagnostic strategies for toxoplasmosis. Clin Microbiol Rev. 2012;25(2):264-296
2. Mattos CC, Meira CS, Ferreira AI, et al. Contribution of laboratory methods in diagnosing clinically suspected ocular toxoplasmosis in Brazilian patients. Diagn Microbiol Infect Dis. 2011;70(3):362-366
3. Jones JL, Kruszon-Moran D, Elder S, et al. Toxoplasma gondii infection in the United States, 2011-2014. Am J Trop Med Hyg. 2018;98(2):551-557. doi:10.4269/ajtmh.17-0677
4. Martino R, Bretagne S, Einsele H, et al. Early detection of Toxoplasma infection by molecular monitoring of Toxoplasma gondii in peripheral blood samples after allogeneic stem cell transplantation. Clin Infect Dis. 2005;40(1):67-78
5. Elsheikha HM, Marra CM, Zhu XQ. Epidemiology, pathophysiology, diagnosis, and management of cerebral toxoplasmosis. Clin Microbiol Rev. 2020;34(1):e00115-19
6. Fricker-Hidalgo H, Bulabois CE, Brenier-Pinchart MP, et al. Diagnosis of toxoplasmosis after allogeneic stem cell transplantation: results of DNA detection and serological techniques. Clin Infect Dis. 2009;48(2):e9-e15
7. Maldonado YA, Read JS. Committee on infectious diseases. Diagnosis, treatment, and prevention of congenital toxoplasmosis in the United States. Pediatrics. 2017;139(2):e20163860. doi:10.1542/peds.2016-3860
Day(s) Performed
Monday through Sunday
Report Available
1 to 4 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
87798
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| TOXB | Toxoplasma gondii PCR, B | 16279-2 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 62977 | Toxoplasma gondii PCR, B | 16279-2 |
Method Name
Polymerase Chain Reaction (PCR)/DNA Probe Hybridization
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.