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Test Code TB2LN Susceptibility, Mycobacterium tuberculosis Complex, Second Line, Varies

Infectious

Useful For

Determination of Mycobacterium tuberculosis complex minimal inhibitory concentrations to second-line antimicrobial agents

Additional Tests

Test ID Reporting Name Available Separately Always Performed
STV2 Susceptibility, Mtb Cx, 2nd Line No, (Bill Only) Yes

Testing Algorithm

When this test is ordered, the additional test will always be performed at an additional charge.

Reporting Name

Susceptibility, Mtb Complex, 2 Line

Specimen Type

Varies


Additional Testing Requirements


If organism identification is not provided, CTB / Mycobacteria and Nocardia Culture, Varies or CTBID / Culture Referred for Identification, Mycobacterium and Nocardia, Varies must also be ordered and will be charged separately.



Shipping Instructions


1. For shipping information see Infectious Specimen Shipping Guidelines.

2. Place specimen in a large infectious container and label as an etiologic agent/infectious substance.



Necessary Information


Specimen source and suspected organism identification are required.



Specimen Required


Specimen Type: Organism

Supplies: Infectious Container, Large (T146)

Container/Tube: Middlebrook 7H10 agar slant

Specimen Volume: Isolate

Collection Instructions: Organism must be in pure culture, actively growing.


Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Ambient (preferred)
  Refrigerated 

Reference Values

Interpretive criteria and reporting guidelines are followed using the Clinical Laboratory Standards Institute (CLSI) M24S document.

Interpretation

Results are reported as minimal inhibitory concentrations in mcg/mL.

 

This test is used as an alternative to TB1LN / Antimicrobial Susceptibility, Mycobacterium tuberculosis Complex, First Line, Varies for ethambutol, isoniazid and rifampin when reagents are not available to perform the TB1LN test. Ethambutol, isoniazid, and rifampin are not routinely reported with this test.

Clinical Reference

1. Hall L, Jude KP, Clark SL, et al. Evaluation of the Sensititre MycoTB plate for susceptibility testing of the Mycobacterium tuberculosis complex against first- and second-line agents. J Clin Microbiol. 2012;50:3732-3734

2. Nahid P, Mase SR, Migliori GB, et al. Treatment of Drug-Resistant Tuberculosis. An Official ATS/CDC/ERS/IDSA Clinical Practice Guideline [published correction appears in Am J Respir Crit Care Med. 2020 Feb 15;201(4):500-501

3. Clinical and Laboratory Standards Institute (CLSI). Susceptibility Testing of Mycobacteria, Nocardia spp., and Other Aerobic Actinomycetes. 3rd ed. CLSI standard M24. CLSI; 2018

4. Clinical and Laboratory Standards Institute (CLSI). Performance Standards for Susceptibility Testing of Mycobacteria, Nocardia spp., and Other Aerobic Actinomycetes. 2nd ed. CLSI supplement M24S. CLSI; 2023

Day(s) Performed

Monday through Sunday

Report Available

21 to 30 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

87186-Susceptibility, Mtb Cx, 2nd Line

LOINC Code Information

Test ID Test Order Name Order LOINC Value
TB2LN Susceptibility, Mtb Complex, 2 Line 29579-0

 

Result ID Test Result Name Result LOINC Value
TB2LN Susceptibility, Mtb Complex, 2 Line 29579-0

Method Name

Minimum Inhibitory Concentration (MIC) by Microtiter Broth Dilution Method

Specimen Minimum Volume

See Specimen Required