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Test Code SHIGC Shigella Culture, Feces


Additional Testing Requirements


If susceptibility testing is needed, also order ZMMLS / Antimicrobial Susceptibility, Aerobic Bacteria, Varies. If susceptibility testing is not needed (eg, due to lack of recovery of Shigella species from feces), it will not be performed and the ZMMLS order will be canceled at time of report.

 

In some cases, local public health requirements may impact Mayo Clinic Laboratories clients, requiring, for example, submission of isolates to public health laboratories. Clients should familiarize themselves with local requirements and are responsible for submitting isolates to appropriate public health laboratories. Clients can obtain isolates of Shigella species recovered from specimens submitted to Mayo Clinic Laboratories by calling 800-533-1710 as soon as possible after reporting (to ensure viability of the bacterium).



Shipping Instructions


Specimen must arrive within 96 hours of collection.



Necessary Information


Specimen source is required.



Specimen Required


Patient Preparation: The patient should not use antacids, barium, bismuth, antidiarrheal medication, or oily laxatives before specimen collection.

Supplies: Culture and Sensitivity Stool Transport Vial (T058)

Container/Tube: Commercially available transport system specific for recovery of enteric pathogens from fecal specimens (15 mL of non-nutritive transport medium containing phenol red as a pH indicator, either Cary-Blair or Para-Pak C and S)

Specimen Volume: Representative portion of fecal specimen

Collection Instructions:

1. Collect fresh feces and submit 1 gram or 5 mL in container with transport medium.

2. Place feces in preservative within 2 hours of collection.

3. Place vial in a sealed plastic bag.


Useful For

Determining whether Shigella species may be the cause of diarrhea

 

Reflexive testing for Shigella species from nucleic acid amplification test-positive stool

 

This test is generally not useful for patients hospitalized more than 3 days because the yield from specimens from these patients is very low, as is the likelihood of identifying a pathogen that has not been detected previously.

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
GID Bacteria Identification No, (Bill Only) No
ISAE Aerobe Ident by Sequencing No, (Bill Only) No
REFID Additional Identification Procedure No, (Bill Only) No
SHIG Serologic Agglut Method 3 Ident No, (Bill Only) No
RMALD Ident by MALDI-TOF mass spec No, (Bill Only) No

Testing Algorithm

When this test is ordered, the reflex tests may be performed at an additional charge.

 

For more information see Laboratory Testing for Infectious Causes of Diarrhea

Method Name

Conventional Culture

Reporting Name

Shigella Culture, F

Specimen Type

Fecal

Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Fecal Ambient (preferred) 4 days
  Refrigerated  4 days

Reference Values

No growth of Shigella species.

Interpretation

The growth of Shigella species identifies a potential cause of diarrhea.

Clinical Reference

1. Pillai DR, Griener T. Culture for Campylobacter and related organisms. In: Leber AL, Church DL, eds. Clinical Microbiology Procedures Handbook. 4th ed. ASM Press; 2016:Section 3.8.2

2. DuPont HL. Persistent diarrhea: A clinical review. JAMA. 2016;315(24):2712-2723. doi:10.1001/jama.2016.7833

3. DuPont HL, Levine MM, Hornick RB, Formal SB. Inoculum size in shigellosis and implications for expected mode of transmission. J Infect Dis. 1989;159(6):1126-1128

4. Maurelli AT, Lampel KA. Shigella. In: Hui YH, Gorham JR, Murrell KD, Cliver DO, eds. Foodborne Disease Handbook. Marcel Dekker;1994:321

5. CDC Health Alert Network. CDC Recommendations for diagnosing and managing Shigella Strains with possible reduced susceptibility to ciprofloxacin. April 18, 2017. Accessed September 22, 2023. Available at https://emergency.cdc.gov/han/han00401.asp

Day(s) Performed

Monday through Friday

Report Available

3 to 5 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

87046-Shigella Culture, Stool-with isolation and preliminary examination

87077-Bacteria Identification (if appropriate)

87153-Aerobe Ident by Sequencing (if appropriate)

87077-Additional Identification Procedure (if appropriate)

87147 x 4-Serologic Agglut Method 3 Ident (if appropriate)

87077-Ident by MALDI-TOF mass spec (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
SHIGC Shigella Culture, F 17576-0

 

Result ID Test Result Name Result LOINC Value
SHIGC Shigella Culture, F 88586-3