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Test Code RPRT3 Rapid Plasma Reagin (RPR) with Reflex to Treponema pallidum Particle Agglutination, Serum


Ordering Guidance


This assay should only be used following an initial reactive treponemal antibody assay result as part of the reverse syphilis testing algorithm.



Specimen Required


Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.3 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Useful For

Diagnosing syphilis

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
RPRT4 RPR, Titer, S No No
RTPPA Syphilis Ab, TP-PA, S Yes, (Order TPPA) No

Testing Algorithm

If this test is positive, then the rapid plasma reagin titer will be performed at an additional charge.

 

If this test is negative, then the Treponema pallidum particle agglutination test will be performed at an additional charge.

 

For more information see Syphilis Serology Algorithm.

Special Instructions

Method Name

RPRT3, RPRT4: Flocculation/Agglutination

RTPPA: Particle Agglutination

Reporting Name

RPR w/ Reflex to TP-PA, S

Specimen Type

Serum

Specimen Minimum Volume

See Specimen Required

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 14 days
  Frozen  14 days

Reference Values

Negative

Reference values apply to all ages.

Interpretation

Negative:

Non-treponemal antibodies not detected. Testing on a new specimen collected in 2 to 3 weeks is recommended if acute infection is suspected. Sample reflexed for detection of Treponema pallidum specific antibodies by the T pallidum particle agglutination assay.

 

Positive:

Specimen reflexed to determine rapid plasma reagin titer.

Clinical Reference

1. Tuddenham S, Katz SS, Ghanem KG: Syphilis Laboratory Guidelines: Performance characteristics of nontreponemal antibody tests. Clin Infect Dis. 2020 Jun 24;71(Suppl 1):S21-S42. doi: 10.1093/cid/ciaa306

2. Park IU, Tran A, Pereira L, Fakile Y: Sensitivity and specificity of treponemal-specific tests for the diagnosis of syphilis. Clin Infect Dis. 2020 Jun 24;71(Suppl 1):S13-S20. doi: 10.1093/cid/ciaa349

3. Theel ES, Katz SS, Pillay A: Molecular and direct detection tests for Treponema pallidum subspecies pallidum: A review of the literature, 1964-2017. Clin Infect Dis. 2020 Jun 24;71(Suppl 1):S4-S12. doi: 10.1093/cid/ciaa176

4. Ortiz DA, Shukla MR, Loeffelholz MJ: The traditional or reverse algorithm for diagnosis of syphilis: Pros and cons. Clin Infect Dis. 2020 Jun 24;71(Suppl 1):S43-S51. doi: 10.1093/cid/ciaa307

Day(s) Performed

Monday through Saturday

Report Available

Same day/1 to 4 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

86592

86593- (if appropriate)

86780- (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
RPRT3 RPR w/ Reflex to TP-PA, S 20507-0

 

Result ID Test Result Name Result LOINC Value
616970 RPR w/ Reflex to TP-PA, S 20507-0

Forms

If not ordering electronically, complete, print, and send an Infectious Disease Serology Test Request (T916) with the specimen.