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HelpTest Code ROPAN Ro52 and Ro60 Antibodies, IgG, Serum
Specimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container: Plastic vial
Specimen Volume: 1 mL
Collection Instructions: Centrifuge and aliquot serum into plastic vial
Useful For
Evaluating patients at risk for connective tissue disease with or without interstitial lung disease
Differentiation of antibodies to Ro52 and Ro60 in patients known to be positive for anti-SS-A (Ro) antibodies
Profile Information
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| RO52 | Ro52 Antibody, IgG, S | Yes | Yes |
| RO60 | Ro60 Antibody, IgG, S | Yes | Yes |
Method Name
Chemiluminescent Immunoassay
Reporting Name
Ro52 and Ro60 Antibodies, IgG, SSpecimen Type
SerumSpecimen Minimum Volume
0.8 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 14 days | |
| Frozen | 21 days |
Reference Values
Ro52 ANTIBODY, IgG
<20 CU (negative)
≥20 CU (positive)
Reference values apply to all ages
Ro60 ANTIBODY, IgG
<20 CU (negative)
≥20 CU (positive)
Reference values apply to all ages
Interpretation
The presence of antibodies to both SS-A 52 (Ro52) or SSA 60 (Ro60) is highly suggestive of a diagnosis of Sjogren syndrome (SS), with positivity for Ro60 alone more likely to be associated with systemic lupus erythematosus (SLE).
Antibodies to Ro52 may be present in patients with idiopathic inflammatory myopathy (IIM), systemic sclerosis (SSc) or overlap connective tissue disease (CTD). In CTD, antibodies to Ro52 alone, or in association with other specific autoantibodies may be associated with interstitial lung diseases.
Negative results for antibodies to Ro52 and Ro60 does not exclude the possibility of any CTD, including SS, SLE, SSc, and IIM.
Clinical Reference
1. Schulte-Pelkum J, Fritzler M, Mahler M: Latest update on the Ro/SS-A autoantibody system. Autoimmun Rev. 2009;8(7):632-637
2. Defendenti C, Atzeni F, Spina MF, et al. Clinical and laboratory aspects of Ro/SSA-52 autoantibodies. Autoimmun Rev. 2010;10(3):150-154
3. Fayyaz A, Kurien BT, Scofield H. Autoantibodies in Sjogren's syndrome. Rheum Dis Clin North Am. 2016;42(3):419-434
4. Armagan B, Robinson SA, Bazoberry A, et al. Antibodies to both ro52 and ro60 for identifying Sjogren's syndrome patients best suited for clinical trials of disease-modifying therapies. Arthritis Care Res (Hoboken). 2022;74(9):1559-1565
5. Robbins A, Hentzien M, Toquet S, et al: Diagnostic utility of separate anti-Ro60 and anti-Ro52/TRIM21 antibody detection in autoimmune diseases. Front Immunol. 2019;10:444
6. Lee AYS, Reed JH, Gordon TP. Anti-Ro60 and anti-Ro52/TRIM21: Two distinct autoantibodies in systemic autoimmune diseases. J Autoimmun. 2021;124:102724
7. Chan EKL. Anti-Ro52 autoantibody is common in systemic autoimmune rheumatic diseases and correlating with worse outcome when associated with interstitial lung disease in systemic sclerosis and autoimmune myositis. Clin Rev Allergy Immunol. 2022;63(2):178-193
8. Gkoutzourelas A, Liaskos C, Mytilinaiou MG, et al. Anti-Ro60 seropositivity determines anti-ro52 epitope mapping in patients with systemic sclerosis. Front Immunol. 2018;9:2835
9. Deroo L, Achten H, De Boeck K, et al. The value of separate detection of anti-Ro52, anti-Ro60 and anti-SSB/La reactivities in relation to diagnosis and phenotypes in primary Sjögren's syndrome. Clin Exp Rheumatol. 2022;40(12):2310-1317
10. Zampeli E, Mavrommati M, Moutsopoulos HM, Skopouli FN. Anti-Ro52 and/or anti-Ro60 immune reactivity: autoantibody and disease associations. Clin Exp Rheumatol. 2020;38 Suppl 126(4):134-141
Day(s) Performed
Wednesday
Report Available
2 to 8 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
86235 x 2
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| ROPAN | Ro52 and Ro60 Antibodies, IgG, S | 33610-7 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| RO52 | Ro52 Antibody, IgG, S | 53017-0 |
| RO60 | Ro60 Antibody, IgG, S | 53019-6 |