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Test Code PTEM Platelet Transmission Electron Microscopic Study, Whole Blood

Useful For

Diagnosing platelet disorders

Method Name

Transmission Electron Microscopy

Reporting Name

Platelet TEM, B

Specimen Type

Whole Blood ACD


Shipping Instructions


Send specimen Monday through Wednesday.

 

Specimens must be received in testing laboratory within 72 hours of collection. Ship specimen overnight in an Ambient Shipping Box-Critical Specimens Only (T668) following the instructions in the mailer.



Necessary Information


Platelet Esoteric Testing Patient Information is required. Testing may proceed without the patient information, however, the information aids in providing a more thorough interpretation. Ordering providers are strongly encouraged to fill out the form and send with the specimen.



Specimen Required


Patient Preparation: Fasting is preferred but not required. 

Supplies: Ambient Shipping Box-Critical Specimens Only (T668)

Collection Container/Tube:

Preferred: Yellow top (ACD, solution B)

Acceptable: Yellow top (ACD, solution A)

Specimen Volume: 6 mL

Collection Instructions: Send whole blood specimen in original tube. Do not aliquot.


Specimen Minimum Volume

3 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Whole Blood ACD Ambient 72 hours

Reference Values

Mean dense granules/platelet: ≥1.2

Interpretation

Ultrastructural abnormalities identified by platelet transmission electron microscopy (TEM) are evaluated by a Mayo hematopathologist.

 

Platelet size, alpha granules, Golgi complex, and abnormal inclusions will be assessed as part of the morphologic examination under TEM.

 

Distinct and sometimes pathognomonic ultrastructural abnormalities are found in Hermansky Pudlak syndrome, gray platelet syndrome with virtually absent alpha granules, white platelet syndrome, Medich giant platelet disorder, X-linked GATA-1 macrothrombocytopenia, and, recently described, York platelet syndrome.

Clinical Reference

1. White JG. Electron-dense chains and clusters in platelets from patients with storage pool-deficiency disorders. J Thromb Haemost. 2003;1(1):74-79. doi:10.1046/j.1538-7836.2003.00032.x

2. White JG. Use of the electron microscope for diagnosis of platelet disorders. Semin Thromb Hemost. 1998;24(2):163-168. doi:10.1055/s-2007-995836

3. Chen D, Uhl CB, Bryant SC, et al. Diagnostic laboratory standardization and validation of platelet transmission electron microscopy. Platelets. 2018;29(6):574-582. doi:10.1080/09537104.2018.1476682

Day(s) Performed

Monday through Friday

Report Available

10 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

85390

88348

LOINC Code Information

Test ID Test Order Name Order LOINC Value
PTEM Platelet TEM, B 79768-8

 

Result ID Test Result Name Result LOINC Value
CK109 Platelet TEM 79768-8
CK110 Interpretation 59466-3

Forms

1. Platelet Esoteric Testing Patient Information is required.

2. If not ordering electronically, complete, print, and send a Coagulation Test Request (T753) with the specimen.