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HelpTest Code PNP Platelet Neutralization Procedure, Plasma
Useful For
Aiding in the confirmation or exclusion of the presence of a lupus anticoagulant (LAC) inhibitor when used in conjunction with other appropriate coagulation tests.
Aids in differentiating deficiencies or inhibitors of specific coagulation factors (eg, factor VIII inhibitor) from LAC inhibitors
Method Name
Only orderable as a reflex. For more information, see:
ALUPP / Lupus Anticoagulant Profile, Plasma
ALBLD / Bleeding Diathesis Profile, Limited, Plasma
AATHR / Thrombophilia Profile, Plasma and Whole Blood
APROL / Prolonged Clot Time Profile, Plasma
ADIC / Disseminated Intravascular Coagulation/Intravascular Coagulation and Fibrinolysis (DIC/ICF) Profile, Plasma
Optical Clot-Based
Reporting Name
Platelet Neutralization ProcedureSpecimen Type
Plasma Na CitSpecimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Plasma Na Cit | Frozen | ||
Reference Values
Only orderable as a reflex. For more information see:
ALUPP / Lupus Anticoagulant Profile, Plasma
ALBLD / Bleeding Diathesis Profile, Limited, Plasma
AATHR / Thrombophilia Profile, Plasma and Whole Blood
APROL / Prolonged Clot Time Profile, Plasma
ADIC / Disseminated Intravascular Coagulation/Intravascular Coagulation and Fibrinolysis (DIC/ICF) Profile, Plasma
An interpretive report will be provided.
Interpretation
Interpretation of the results of the platelet neutralization procedure (PNP) test is complex and needs to be performed in the context of results of mixing study of the prolonged activated partial thromboplastin time (APTT), the APTT PNP and the buffer control APTT, as well as results of other coagulation tests (eg, prothrombin time and thrombin time as well as available clinical information).
Plasma containing lupus anticoagulant (LAC) will demonstrate shortening of the PNP Platelets APTT by 2 or more seconds when compared to the baseline PNP Saline APTT.
Clinical Reference
1. Brandt JT, Triplett DA, Alving B, Scharrer I. Criteria for the diagnosis of lupus anticoagulants: an update. Thromb Haemost. 1995;74(5):1185-1190
2. Brandt JT, Barna LK, Triplett DA. Laboratory identification of lupus anticoagulants: results of the second international workshop for identification of lupus anticoagulants. Thromb Haemost. 1995;74(6):1597-1603
3. Gastineau DA, Kazmier FJ, Nichols WL, Bowie EJ. Lupus anticoagulant: an analysis of the clinical and laboratory features of 219 cases. Am J Hematol. 1985;19(3):265-275
4. Cardel LK, Fisher PK, Heit JA, et al. Detection of lupus anticoagulants and anticardiolipin antibodies: prevalence of positive test in 665 patients. Thromb Haemost. 1993;69:1221
5. Kottke-Marchant K, Davis BH. Laboratory Hematology Practice. Wiley Blackwell Publishing; 2012
6. Clinical and Laboratory Standards Institute (CLSI). Laboratory Testing for the Lupus Anticoagulant; Approved Guideline. CLSI document H60-A. CLSI; 2014
Day(s) Performed
Monday through Friday
Report Available
1 to 4 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| PNP | Platelet Neutralization Procedure | 103620-1 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| PNPPL | Platelet Neutralization Procedure | 75506-6 |
| PNPSA | PNP Buffer Control | 103619-3 |
Specimen Required
Only orderable as a reflex. For more information see:
ALUPP / Lupus Anticoagulant Profile, Plasma
ALBLD / Bleeding Diathesis Profile, Limited, Plasma
AATHR / Thrombophilia Profile, Plasma and Whole Blood
APROL / Prolonged Clot Time Profile, Plasma
ADIC / Disseminated Intravascular Coagulation/Intravascular Coagulation and Fibrinolysis (DIC/ICF) Profile, Plasma
Specimen Stability Information: Frozen 2 years
Specimen Minimum Volume
See Specimen Required
CPT Code Information
85597