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Test Code PEANT Peanut, IgE with Reflex to Peanut Components, IgE, Serum

Useful For

Evaluating patients with suspected peanut allergy

 

Evaluating patients with possible peanut cross-reactivity

 

This test is not useful for patients previously treated with immunotherapy to determine if residual clinical sensitivity exists or in whom the medical management does not depend upon identification of allergen specificity.

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
PNTCP Peanut Components, IgE, S No No

Testing Algorithm

Testing begins with analysis of peanut IgE. If peanut IgE is negative (<0.10 kU/L), testing is complete.

 

If peanut IgE is 0.10 kU/L or greater, then 7 peanut components (Ara h 2, Ara h 1, Ara h 3, Ara h 6, Ara h 8, Ara h 9, and profilin Bet v2) are performed at an additional charge.

Method Name

Fluorescent Enzyme Immunoassay (FEIA)

Reporting Name

Peanut Component Reflex, S

Specimen Type

Serum


Ordering Guidance


For a listing of allergens available for testing, see Allergens - Immunoglobulin E (IgE) Antibodies.



Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 2 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 14 days
  Frozen  90 days
  Ambient  7 days

Reference Values

Class

IgE kU/L

Interpretation

0

<0.10

Negative

0/1

0.10-0.34

Borderline / Equivocal

1

0.35-0.69

Equivocal

2

0.70-3.49

Positive

3

3.50-17.4

Positive

4

17.5-49.9

Strongly positive

5

50.0-99.9

Strongly positive

6

≥100

Strongly positive

 

Concentrations of 0.70 kU/L or more (class 2 and above) will flag as abnormally high.

Reference values apply to all ages.

Interpretation

When detectable total peanut IgE antibody is present (≥0.10 IgE kU/L), additional specific component IgE antibody testing will be performed. If at least one potential specific allergenic peanut component IgE is detectable (≥0.10 IgE kU/L), an interpretative report will be provided.

 

When the sample is negative for total peanut IgE antibody (<0.10 IgE kU/L), further testing for specific peanut component IgE antibodies will not be performed. Negative IgE results for total peanut antibody may indicate a lack of sensitization to potential peanut allergenic components.

Clinical Reference

1. Sicherer SH, Wood RA. Advances in diagnosing peanut allergy. J Allergy Clin Immunol Pract. 2013;1(1):1-14. doi:10.1016/j.jaip.2012.10.004

2. Eller E, Bindslev-Jensen C. Clinical value of component-resolved diagnostics in peanut-allergic patients. Allergy. 2013;68(2):190-194. doi:10.1111/all.12075

3. Hong X, Caruso D, Kumar R, et al. IgE, but not IgG4, antibodies to Ara h 2 distinguish peanut allergy from asymptomatic peanut sensitization. Allergy. 2012;67(12):1538-1546. doi:10.1111/all.12047

4. Asarnoj A, Nilsson C, Lidholm J, et al. Peanut component Ara h 8 sensitization and tolerance to peanut. J Allergy Clin Immunol. 2012;130(2):468-472. doi:10.1016/j.jaci.2012.05.019

5. Klemans RJ, van Os-Medendorp H, Blankestijn M, Bruijnzeel-Koomen CA, Knol EF, Knulst AC. Diagnostic accuracy of specific IgE to components in diagnosing peanut allergy: a systematic review. Clin Exp Allergy. 2015;45(4):720-730. doi:10.1111/cea.12412

6. Koid AE, Chapman MD, Hamilton RG, et al. Ara h 6 complements Ara h 2 as an important marker for IgE reactivity to peanut. J Agric Food Chem. 2014;62(1):206-213. doi:10.1021/jf4022509

7. Bublin M, Breiteneder H. Cross-reactivity of peanut allergens. Curr Allergy Asthma Rep. 2014;14(4):426. doi:10.1007/s11882-014-0426-8

8. Chan ES, Greenhawt MJ, Fleischer DM, Caubet JC. Managing cross-reactivity in those with peanut allergy. J Allergy Clin Immunol Pract. 2019;7(2):381-386. doi:10.1016/j.jaip.2018.11.012

9. Simberloff T, Parambi R, Bartnikas LM, et al. Implementation of a standardized clinical assessment and management plan (SCAMP) for food challenges. J Allergy Clin Immunol Pract. 2017;5(2):335-344.e3. doi:10.1016/j.jaip.2016.05.021

Day(s) Performed

Monday through Friday

Report Available

Same day/1 to 3 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

86003

LOINC Code Information

Test ID Test Order Name Order LOINC Value
PEANT Peanut Component Reflex, S 6206-7

 

Result ID Test Result Name Result LOINC Value
PNUT Peanut, IgE, S 6206-7

Forms

If not ordering electronically, complete, print, and send an Allergen Test Request (T236) with the specimen.