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HelpTest Code PBORB Lyme Disease, Molecular Detection, PCR, Blood
Reporting Name
Lyme Disease PCR, BUseful For
Supporting the diagnosis of Lyme disease in conjunction with serologic testing
This test should not be used to screen asymptomatic patients.
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
Whole Blood EDTAOrdering Guidance
This assay does not detect Borrelia miyamotoi. If infection with this organism is suspected, order BMIPB / Borrelia miyamotoi Detection, PCR, Blood or BMIYC / Borrelia miyamotoi Detection, PCR, Spinal Fluid.
Specimen Required
Container/Tube: Lavender top (EDTA)
Specimen Volume: 1 mL
Collection Instructions: Send whole blood specimen in original tube. Do not aliquot.
Specimen Minimum Volume
0.3 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Whole Blood EDTA | Refrigerated (preferred) | 7 days |
| Frozen | 7 days |
Reference Values
Negative
Reference values apply to all ages.
Day(s) Performed
Monday through Sunday
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
87476
87798 x 2
87999 (if appropriate for government payers)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| PBORB | Lyme Disease PCR, B | 90892-1 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 56080 | B. burgdorferi PCR, B | 94247-4 |
| 38290 | B. mayonii PCR, B | 94248-2 |
| 38291 | B. garinii/B. afzelii PCR, B | 94249-0 |
| 38340 | Lyme Disease PCR Comment | 59464-8 |
Interpretation
A positive result indicates the presence of DNA from Borrelia burgdorferi, Borrelia mayonii, Borrelia afzelii, or Borrelia garinii, the main agents of Lyme disease.
A negative result indicates the absence of detectable target DNA in the specimen. Due to the diagnostic sensitivity limitations of the polymerase chain reaction assay, a negative result does not preclude the presence of the organism or active Lyme disease.
Clinical Reference
1. Stanek G, Wormser GP, Gray J, Strle F. Lyme borreliosis. Lancet. 2012;379(9814):461-473
2. Pritt BS, Mead PS, Johnson, DK, et al. Identification of a novel pathogenic Borrelia species causing Lyme borreliosis with unusually high levels of spirochetemia: a descriptive study. Lancet Infect Dis. 2016;16(5):556-564
3. Pritt BS, Respicio-Kingry LB, Sloan LM, et al. Borrelia mayonii sp. nov., a member of the Borrelia burgdorferi sensu lato complex, detected in patients and ticks in the upper midwestern United States. Int J Sys Evol Microbiol. 2016;66(11):4878-4880
4. Hinckley AF, Connally NP, Meek JI, et al. Lyme disease testing by large commercial laboratories in the United States. Clin Infect Dis. 2014;59(5):676-681
5. Lindgren E, Jaenson TGT. Lyme borreliosis in Europe: influences of climate and climate change, epidemiology, ecology and adaptation measures. World Health Organization; 2006
6. Centers for Disease Control and Prevention. Recommendations for test performance and interpretation from the Second National Conference on Serologic Diagnosis of Lyme Disease. MMWR Morb Mortal Wkly Rep. 1995 Aug;44(31):590-591
7. Babady NE, Sloan LM, Vetter EA, et al. Percent positive rate of Lyme real-time polymerase chain reaction in blood, cerebrospinal fluid, synovial fluid, and tissue. Diagn Microbiol Infect Dis. 2008;62(4):464-466
8. Centers for Disease Control and Prevention (CDC). Lyme disease-United States, 1995. MMWR Morb Mortal Wkly Rep. 1996;45(23):481-484
Report Available
1 to 4 daysMethod Name
Real-Time Polymerase Chain Reaction (PCR)/DNA Probe Hybridization
Testing Algorithm
For more information see Acute Tickborne Disease Testing Algorithm.
Special Instructions
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.