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Test Code PAI1 Plasminogen Activator Inhibitor Antigen, Plasma


Specimen Required


Specimen Type: Platelet-poor plasma

Collection Container/Tube: Light-blue top (3.2% sodium citrate)

Submission Container/Tube: Plastic vial (polypropylene preferred)

Specimen Volume: 1 mL

Collection Instructions:

1. For complete instructions, see Coagulation Guidelines for Specimen Handling and Processing.

2. Centrifuge, transfer all plasma into a plastic vial, and centrifuge plasma again.

3. Aliquot plasma into a plastic vial leaving 0.25 mL in the bottom of centrifuged vial.

4. Freeze plasma immediately (no longer than 4 hours after collection) at -20° C or ideally, at or below -40° C.

Specimen Stability Information: Frozen 2 years

Additional Information:

1. Double-centrifuged specimen is critical for accurate results as platelet contamination may cause spurious results.

2. Each coagulation assay requested should have its own vial.


Useful For

Identification of heredity elevation or deficiency of plasminogen activator inhibitor type 1

 

Determination of risk for veno-occlusive disease associated with bone marrow transplantation

 

Differential diagnosis of impaired fibrinolysis

 

Prognostic marker of occurrence or recurrence of thrombosis

Method Name

Enzyme-Linked Immunosorbent Assay (ELISA)

Reporting Name

PAI-1 Ag, P

Specimen Type

Plasma Na Cit

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Plasma Na Cit Frozen

Reference Values

3.0-72.0 ng/mL

Interpretation

Increased levels of plasminogen activator inhibitor type 1 (PAI-1) are associated with a predisposition to thrombosis.

 

Decreased or absent levels of detectable functional PAI-1 will result in a life-long bleeding diathesis.

Clinical Reference

1. Lee JH, Lee KH, Lee JH, et al. Plasminogen activator inhibitor-1 is an independent diagnostic marker as well as severity predictor of hepatic veno-occlusive disease after allogeneic bone marrow transplantation in adults conditioned with busulphan and cyclophosphamide. Br J Haematol. 2002;118(4):1087-1094

2. Stiko A, Hervio L, Loskutoff DJ. Plasminogen activator inhibitors. In: Colman RW, Hirsh J, Marder VJ, et al, eds. Hemostasis and Thrombosis. Lippincott; 2001:355-365

3. Vaughn DE, Declerck PJ. Regulation of fibrinolysis. In: Loscalzo J, Schager A, eds. Thrombosis and Hemorrhage. Lippincott; 2003:389-396

4. Goodnight SH Jr, Hathaway WE. Fibrinolytic defects and thrombosis. In: Disorders of Hemostasis and Thrombosis: A Clinical Guide. McGraw-Hill Book Company; 2001:389-396

5. Kruithof EK, Gudinchet A, Bachman F. Plasminogen activator inhibitor-1 and plasminogen activator inhibitor-2 in various disease states. Thromb Haemostasis. 1988;59(1):7-12

6. Salat C, Holler E, Kolb HJ, et al. Plasminogen activator inhibitor-1 confirms the diagnosis of hepatic veno-occlusive disease in patients with hyperbilirubinemia after bone marrow transplantation. Blood. 1997;89(6):2184-2188

7. Fay WP, Shapiro AD, Shih JL, Schleef RR, Ginsburg D. Brief report: complete deficiency of plasminogen-activator inhibitor type 1 due to a frame-shift mutation. N Engl J Med. 1992;327(24):1729-1733

8. Heiman M, Gupta S, Khan SS, et al. Complete plasminogen activator inhibitor 1 deficiency. In: Adam MP, Ardinger HH, Pagon RA, et al, eds. GeneReviews [Internet]; 2017. Updated February 23, 2023. Accessed February 21, 2024. Available at www.ncbi.nlm.nih.gov/books/NBK447152/

Day(s) Performed

Wednesday

Report Available

7 to 12 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

85415

LOINC Code Information

Test ID Test Order Name Order LOINC Value
PAI1 PAI-1 Ag, P 22758-7

 

Result ID Test Result Name Result LOINC Value
86083 PAI-1 Ag, P 22758-7