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Test Code P5NT Pyrimidine 5' Nucleotidase, Blood

Reporting Name

Pyrimidine 5' Nucleotidase, B

Useful For

Evaluation of marked basophilic stippling

 

Evaluation of hemolytic anemia

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Whole Blood ACD-B


Specimen Required


Collection Container/Tube:

Preferred: Yellow top (ACD solution B)

Acceptable: Lavender top (EDTA)

Specimen Volume: 5 mL

Collection Instructions: Send whole blood specimen in original tube. Do not aliquot.


Specimen Minimum Volume

3 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Whole Blood ACD-B Refrigerated 20 days

Reference Values

Normal

Day(s) Performed

Monday through Friday

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

83915

LOINC Code Information

Test ID Test Order Name Order LOINC Value
P5NT Pyrimidine 5' Nucleotidase, B 2902-5

 

Result ID Test Result Name Result LOINC Value
2734 Pyrimidine 5' Nucleotidase, B 2902-5

Interpretation

A normal result indicates the absence of pyrimidine nucleotides and indicates normal pyrimidine 5' nucleotidase (P5'N) function.

 

An abnormal result (abnormal spectral scan) indicates the presence of pyrimidine nucleotides and possible P5'N deficiency. Enzyme activity is inhibited by heavy metal ions, including lead, mercury, copper, nickel, and cadmium. Toxic levels can cause accumulation of intracellular pyrimidine nucleotides. If results are abnormal clinical correlation is recommended to exclude heavy metal poisoning.

Clinical Reference

1. Rees DC, Duley JA, Marinaki AM. Pyrimidine 5' nucleotidase deficiency. Br J Haematol. 2003;120(3):375-383

2. Zanella A, Bianchi P, Fermo E, Valentini G. Hereditary pyrimidine 5'-nucleotidase deficiency: from genetics to clinical manifestations. Br J Haematol. 2006;133(2):113-123

3. Fairbanks VF, Klee GG. Biochemical aspects of hematology. In: Burtis CA, Ashwood ER, eds. Tietz Textbook of Clinical Chemistry. 3rd ed. WB Saunders; 1999:1642-1647

4. Gregg XT, Prchal JT. Red blood cell enzymopathies. In: Hoffman R, Benz, Jr EJ, et al, eds. Hematology : Basic Principles and Practice, 7th ed. Elsevier; 2018:616-625

5. Warang P, Colah R, Kedar P: Lead poisoning induced severe hemolytic anemia, basophilic stippling, mimicking erythrocyte pyrimidine 5'-nucleotidase deficiency in beta thalassemia minor. J Clin Toxicol. 2017;7(2):1000346. doi: 10.4172/2161-0495.1000346

Report Available

10 days

Method Name

Kinetic Spectrophotometry

Forms

If not ordering electronically, complete, print, and send a Benign Hematology Test Request Form (T755) with the specimen.