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Test Code NSYPH Syphilis IgG Enzyme Immunoassay, Serum


Specimen Required


Collection Container/Tube: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.6 mL

Collection Instructions: Centrifuge and aliquot serum into plastic vial.


Useful For

An aid in the diagnosis of infection with Treponema pallidum

 

Routine prenatal screening

 

This test is not useful for diagnosis of congenital syphilis.

 

This test is not offered as a screening or confirmatory test for blood donor specimens.

Method Name

Enzyme Immunoassay (EIA)

Reporting Name

Syphilis IgG EIA, S

Specimen Type

Serum

Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Frozen 14 days

Reference Values

Nonreactive

Reference values apply to all ages

Interpretation

Nonreactive:

No serologic evidence of exposure to Treponema pallidum (syphilis). Repeat testing may be considered in patients with suspected acute or primary syphilis.

 

Equivocal:

Recommend follow-up testing in 10 to 14 days if clinically indicated.

 

Reactive:

Results suggest infection with T pallidum at some point in time. Results do not distinguish between recent or past infection, or between treated and untreated syphilis as treponema-specific IgG may remain elevated despite appropriate therapy. Falsely reactive treponemal results may occur; additional testing by a non-treponemal assay is recommended if not previously performed on this sample.

Clinical Reference

1. Centers for Disease Control and Prevention (CDC): Discordant results from reverse sequence syphilis screening-five laboratories, United States, 2006-2010. Morb Mortal WKLY Rep. 2011 Feb 11;60(5):133-137

2. Radolf JD, Tramont EC, Salazar JC: Syphilis (Treponema pallidum). In: Bennett JE, Dolin R, Blaser MJ, eds. Mandell, Douglas, and Bennett's Principles and Practice of Infectious Diseases. 9th ed. Elsevier; 2020:2865-289291

3. Binnicker MJ, Jespersen DJ, Rollins LO: Direct comparison of the traditional and reverse syphilis screening algorithms in a population with a low prevalence of syphilis. J Clin Microbiol. 2012 Jan;50(1):148-150

Day(s) Performed

Monday through Friday, Sunday

Report Available

Same day/1 to 3 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

86780

LOINC Code Information

Test ID Test Order Name Order LOINC Value
NSYPH Syphilis IgG EIA, S 47238-1

 

Result ID Test Result Name Result LOINC Value
NSYPH Syphilis IgG EIA, S 47238-1

Forms

If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.