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Test Code MUXF3 MUXF3 (Cross-reactive Carbohydrate Determinant), IgE, Serum


Ordering Guidance


For a listing of allergens available for testing, see Allergens - Immunoglobulin E (IgE) Antibodies



Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL for every 5 allergens requested

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Forms

If not ordering electronically, complete, print, and send an Allergen Test Request (T236) with the specimen.

Useful For

Evaluation for the presence of antibodies to cross-reactive carbohydrate determinates

 

Investigation of clinically unexpected positive IgE antibody testing in a wide variety of plant and invertebrate allergens

 

This test is not useful for patients previously treated with immunotherapy to determine if residual clinical sensitivity exists or in patients in whom the medical management does not depend upon identification of allergen specificity.

Method Name

Fluorescence Enzyme Immunoassay (FEIA)

Reporting Name

MUXF3 (CCD), IgE, S

Specimen Type

Serum

Specimen Minimum Volume

For 1 allergen: 0.3 mL
For more than 1 allergen: (0.05 mL x number of allergens) + 0.25 mL deadspace

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 14 days
  Frozen  90 days
  Ambient  7 days

Reference Values

Class

IgE kU/L

Interpretation

0

<0.10

Negative

0/1

0.10-0.34

Borderline/equivocal

1

0.35-0.69

Equivocal

2

0.70-3.49

Positive

3

3.50-17.4

Positive

4

17.5-49.9

Strongly positive

5

50.0-99.9

Strongly positive

6

≥100

Strongly positive

Reference values apply to all ages.

 

Concentrations of 0.70 kU/L or more (class 2 and above) will flag as abnormally high.

Interpretation

Antibody to bromelain MUXF3 has widely been used for assessing for potential cross-reactive carbohydrate determinate (CCD) cross-reactivity since its CCD chain is also found in many other plant proteins, including peanuts. While sensitization to CCD is generally not associated with an allergic reaction, the presence of IgE antibodies to CCD may give rise to confounding positive IgE antibody sensitization profiles for a wide variety of plant and invertebrate allergens.

Clinical Reference

1. Altmann F. Coping with cross-reactive carbohydrate determinants in allergy diagnosis. Allergo J Int. 2016;25(4):98-105. doi:10.1007/s40629-016-0115-3

2. Hemmer W, Altmann F, Holzweber F, Gruber C, Wantke F, Wohrl S. ImmunoCAP cellulose displays cross-reactive carbohydrate determinant (CCD) epitopes and can cause false-positive test results in patients with high anti-CCD IgE antibody levels. J Allergy Clin Immunol. 2018;141(1):372-381.e3. doi:10.1016/j.jaci.2017.04.028

3. Sinson E, Ocampo C, Liao C, et al. Cross-reactive carbohydrate determinant interference in cellulose-based IgE allergy tests utilizing recombinant allergen components. PLoS One. 2020;15(4):e0231344. doi:10.1371/journal.pone.0231344

4. van Ree R. Clinical importance of cross-reactivity in food allergy. Curr Opin Allergy Clin Immunol. 2004;4:235-40

5. Fotish K, Altmann F, Haustein D, Vieths S. Involvement of carbohydrate epitopes in the IgE response of celery-allergic patients. Int Arch Allergy Immunol. 1999;120:30-42. doi:10.1159/000024217

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

86008

LOINC Code Information

Test ID Test Order Name Order LOINC Value
MUXF3 MUXF3 (CCD), IgE, S 58984-6

 

Result ID Test Result Name Result LOINC Value
MUXF3 MUXF3 (CCD), IgE, S 58984-6

Day(s) Performed

Monday through Friday

Report Available

Same day/1 to 3 days