Test Code MUXF3 MUXF3 (Cross-reactive Carbohydrate Determinant), IgE, Serum
Ordering Guidance
For a listing of allergens available for testing, see Allergens - Immunoglobulin E (IgE) Antibodies
Specimen Required
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL for every 5 allergens requested
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Forms
If not ordering electronically, complete, print, and send an Allergen Test Request (T236) with the specimen.
Useful For
Evaluation for the presence of antibodies to cross-reactive carbohydrate determinates (CCD)
Investigation of clinically unexpected positive IgE antibody testing in a wide variety of plant and invertebrate allergens
Special Instructions
Method Name
Fluorescence Enzyme Immunoassay (FEIA)
Reporting Name
MUXF3 (CCD), IgE, SSpecimen Type
SerumSpecimen Minimum Volume
For 1 allergen: 0.3 mL
For more than 1 allergen: (0.05 mL x number of allergens) + 0.25 mL deadspace
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Serum | Refrigerated (preferred) | 14 days | |
Frozen | 90 days | ||
Ambient | 7 days |
Reference Values
Class |
IgE kU/L |
Interpretation |
0 |
<0.10 |
Negative |
0/1 |
0.10-0.34 |
Borderline/equivocal |
1 |
0.35-0.69 |
Equivocal |
2 |
0.70-3.49 |
Positive |
3 |
3.50-17.4 |
Positive |
4 |
17.5-49.9 |
Strongly positive |
5 |
50.0-99.9 |
Strongly positive |
6 |
≥100 |
Strongly positive |
Concentrations of 0.70 kU/L or more (class 2 and above) will flag as abnormally high.
Reference values apply to all ages.
Interpretation
Antibody to bromelain MUXF3 has widely been used for assessing for potential cross-reactive carbohydrate determinate (CCD) cross-reactivity since its CCD chain is also found in many other plant proteins, including peanuts. While sensitization to CCD is generally not associated with an allergic reaction, the presence of IgE antibodies to CCD may give rise to confounding positive IgE antibody sensitization profiles for a wide variety of plant and invertebrate allergens.
Clinical Reference
1. Altmann F: Coping with cross-reactive carbohydrate determinants in allergy diagnosis. Allergo J Int. 2016;25(4):98-105. doi:10.1007/s40629-016-0115-3
2. Hemmer W, Altmann F, Holzweber F, Gruber C, Wantke F, Wohrl S: ImmunoCAP cellulose displays cross-reactive carbohydrate determinant (CCD) epitopes and can cause false-positive test results in patients with high anti-CCD IgE antibody levels. J Allergy Clin Immunol. 2018 Jan;141(1):372-381.e3. doi: 10.1016/j.jaci.2017.04.028
3. Sinson E, Ocampo C, Liao C, et al: Cross-reactive carbohydrate determinant interference in cellulose-based IgE allergy tests utilizing recombinant allergen components. PLoS One. 2020 Apr 23;15(4):e0231344. doi: 10.1371/journal.pone.0231344
4. van Ree R. Clinical importance of cross-reactivity in food allergy. Curr Opin Allergy Clin Immunol. 2004;4:235-40
5. Fotish K, Altmann F, Haustein D, Vieths S: Involvement of carbohydrate epitopes in the IgE response of celery-allergic patients. Int Arch Allergy Immunol. 1999 Sep;120:30-42. doi: 10.1159/000024217
Performing Laboratory
Mayo Clinic Laboratories in RochesterTest Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
86008
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
MUXF3 | MUXF3 (CCD), IgE, S | 58984-6 |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
MUXF3 | MUXF3 (CCD), IgE, S | 58984-6 |
Day(s) Performed
Monday through Friday