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HelpTest Code MTBS Microsporidia Stain, Varies
Reporting Name
Microsporidia StainUseful For
Diagnosis of extra-intestinal microsporidiosis involving the lung, skin, and other organs, particularly in immunocompromised hosts
Diagnosis of ocular microsporidiosis
Testing Algorithm
For more information see Parasitic Investigation of Stool Specimens Algorithm
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
VariesOrdering Guidance
This test is intended to be ordered on specimens other than stool and urine.
Specimen Required
Submit only 1 of the following specimens:
Specimen Type: Duodenal aspirate (small intestinal aspirate, jejunal aspirate, small bowel aspirate)
Container/Tube: Sterile container
Specimen Volume: 0.5 mL
Additional Information: ECOFIX and 10% formalin are acceptable preservatives.
Specimen Stability Information: Preserved Ambient (preferred) <10 days/Refrigerated <3 days
Specimen Type: Respiratory secretions (bronchoalveolar lavage [BAL], sputum, bronchial wash, pleural fluid)
Container/Tube: Sterile container
Specimen Volume: 0.5 mL
Specimen Stability Information: Refrigerated <3 days (preferred)/Frozen <10 days
Specimen Type: Eye (vitreous fluid, ocular fluid)
Container/Tube: Sterile container
Specimen Volume: 0.5 mL
Specimen Stability: Refrigerated <3 days
Specimen Type: Corneal scraping
Container/Tube: Sterile container
Specimen Volume: 0.5mL in sterile saline
Additional Information: Place scrapings in sterile saline for shipping.
Specimen Stability: Refrigerated <3 days
Specimen Type: Fresh tissue (lung, eye, bladder, rectal, intestinal, colon, skin, muscle, kidney)
Container/Tube: Sterile container
Specimen Volume: 3-mm biopsy in 0.1-mL sterile saline
Specimen Stability: Refrigerated <3 days
Specimen Type: Gallbladder aspirate/Bile aspirate
Container/Tube: Sterile container
Specimen Volume: 0.5 mL
Specimen Stability: Refrigerated <3 days/Frozen <10 days
Specimen Minimum Volume
See Specimen Required
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Varies | ||
Special Instructions
Reference Values
Negative
If positive, reported as Microsporidia detected
Day(s) Performed
Monday through Friday
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
87015-Concentration
87207-Stain
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| MTBS | Microsporidia Stain | 10857-1 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| MTBS | Microsporidia Stain | 10857-1 |
Interpretation
A positive result suggests an active or recent infection. Results should be correlated with the patient's clinical presentation and immune status.
A negative result indicates absence of detectable microsporidial spores in the specimen but does not always rule out ongoing microsporidiosis since the organism may be present at very low levels or shed sporadically.
Clinical Reference
1. Weber R, Bryan RT, Schwartz DA, Owen RL. Human microsporidial infections. Clin Microbiol Rev. 1994;7:426-461
2. Goodgame RW. Understanding intestinal spore-forming protozoa: cryptosporidia, microsporidia, isospora, and cyclospora. Ann Intern Med. 1996;124:429-441
3. Wanke CA, DeGirolami P, Federman M. Enterocytozoon bieneusi infection and diarrheal disease in patients who were not infected with human immunodeficiency virus: case report and review. Clin Infect Dis. 1996;23:816-818
4. Special Stains for Microsporidia: Modified Trichrome-Ryan Blue. American Society of Microbiology. Updated December 19, 2022. Accessed August 31, 2023. Available at www.clinmicronow.org/doi/10.1128/9781683670438.CMPH.ch9.4-4
5. Special Stains for Microsporidia: Modified Trichrome-Ryan Blue. Center for Disease Control and Prevention. Updated May 29, 2019. Accessed August 31, 2023. Available at www.cdc.gov/dpdx/microsporidiosis/index.html
Report Available
2 to 4 daysMethod Name
Trichrome-Blue Stain (Ryan Modification)
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.