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HelpTest Code MPO Myeloperoxidase Antibodies, IgG, Serum
Reporting Name
Myeloperoxidase Ab, SUseful For
Evaluating patients with clinical features anti-neutrophil cytoplasmic antibody associated vasculitis, specifically granulomatosis with polyangiitis, microscopic polyangiitis (MPA), and eosinophilic granulomatosis with polyangiitis
Distinguishing between MPA and other forms of ANCA-associated vasculitis, in conjunction with proteinase 3 antibody and cytoplasmic neutrophil antibody testing
Following treatment response or monitoring disease activity in patients with myeloperoxidase (MPO) antibodies
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
SerumAdditional Testing Requirements
To distinguish between microscopic polyangiitis and other forms of anti-neutrophil cytoplasmic antibody-associated vasculitis, also order PR3 / Proteinase 3 Antibodies, IgG, Serum and ANCA / Cytoplasmic Neutrophil Antibodies, Serum; alternatively, VASC / Antineutrophil Cytoplasmic Antibodies Vasculitis Panel, Serum may be ordered instead, which initially tests for myeloperoxidase and proteinase 3 antibodies, with reflex to ANCA when appropriate.
Specimen Required
Supplies: Sarstedt Aliquot Tube 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Specimen Minimum Volume
0.35 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 21 days | |
| Frozen | 21 days |
Reference Values
<0.4 U (negative)
0.4-0.9 U (equivocal)
≥1.0 U (positive)
Reference values apply to all ages.
Day(s) Performed
Monday through Saturday
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
83516
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| MPO | Myeloperoxidase Ab, S | 48404-8 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| MPO | Myeloperoxidase Ab, S | 48404-8 |
Interpretation
Positive results for myeloperoxidase (MPO)-antineutrophil cytoplasmic antibodies (ANCA) by antigen-specific immunoassay and perinuclear ANCA by indirect immunofluorescence are consistent with the diagnosis of microscopic polyangiitis (MPA) or eosinophilic granulomatosis with polyangiitis, in patients with the appropriate clinical presentation.
The reactivity of MPO-ANCA may decline with treatment of patients with MPA.
Clinical Reference
1. Kitching AR, Anders HJ, Basu N, et al. ANCA-associated vasculitis. Nat Rev Dis Primers. 2020;6(1):71
2. Ramponi G, Folci M, De Santis M, et al. The biology, pathogenetic role, clinical implications, and open issues of serum anti-neutrophil cytoplasmic antibodies. Autoimmun Rev. 2021;20(3):102759
3. Bossuyt X, Cohen Tervaert W, Arimura Y, et al. Revised 2017 international consensus on testing of ANCAs in granulomatosis with polyangiitis and microscopic polyangiitis. Nat Rev Rheumatol. 2017;13(11):683-692
4. Suppiah R, Robson JC, Grayson PC, et al. 2022 American College of Rheumatology/European Alliance of Associations for Rheumatology classification criteria for microscopic polyangiitis. Ann Rheum Dis. 2022;81(3):321-326. doi:10.1136/annrheumdis-2021-221796
5. Robson JC, Grayson PC, Ponte C, et al. 2022 American College of Rheumatology/European Alliance of Associations for Rheumatology classification criteria for granulomatosis with polyangiitis. Ann Rheum Dis. 2022;81(3):315-320. doi:10.1136/annrheumdis-2021-221795
6. Grayson PC, Ponte C, Suppiah R, et al. 2022 American College of Rheumatology/European Alliance of Associations for Rheumatology classification criteria for eosinophilic granulomatosis with polyangiitis. Ann Rheum Dis. 2022;81(3):309-314. doi:10.1136/annrheumdis-2021-221794
Report Available
2 to 4 daysMethod Name
Multiplex Flow Immunoassay
Forms
If not ordering electronically, complete, print, and send a Renal Diagnostics Test Request (T830) with the specimen.