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Test Code MONOF Monocyte Repartition by CD14/CD16, Blood


Ordering Guidance


If cytogenetic tests are also desired an additional specimen should be submitted. It is important that the specimen be obtained, processed, and transported according to instructions for the other required test.



Shipping Instructions


Specimens must be refrigerated and received within 3 days of collection.



Specimen Required


Container/Tube:

Preferred: Yellow top (ACD solution A or B)

Acceptable: Lavender top (EDTA)

Specimen Volume: 3 mL

Collection Instructions: Send whole blood specimen in original tube. Do not aliquot.


Useful For

Aiding in the diagnosis and monitoring of chronic myelomonocytic leukemia

Method Name

Immunophenotyping

Reporting Name

Monocyte Repartition by CD14/CD16,B

Specimen Type

Whole blood

Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Whole blood Refrigerated 72 hours

Reference Values

% Monocytes of WBC: 1.0-6.6%

MO1 (classical monocytes): <94.0%

Interpretation

An interpretive report describing the classical monocytes (MO1) fraction as either increased or normal will be provided. See Cautions.

Clinical Reference

1. Selimoglu-Buet D, Wagner-Ballon O, Saada V, et al. Characteristic repartition of monocyte subsets as a diagnostic signature of chronic myelomonocytic leukemia. Blood. 2015;125(23):3618-3626. doi:10.1182/blood-2015-01-620781

2. Pophali PA, Marinelli LM, Ketterling RP, et al. High level MYC amplification in B-cell lymphomas: is it a marker of aggressive disease? Blood Cancer J. 2020;10(1):5. doi:10.1038/s41408-019-0271-z

3. Patnaik MM, Timm MM, Vallapureddy R, et al. Flow cytometry based monocyte subset analysis accurately distinguishes chronic myelomonocytic leukemia from myeloproliferative neoplasms with associated monocytosis. Blood Cancer J. 2017;7(7):e584. doi:10.1038/bcj.2017.66

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

88184-Flow cytometry; first cell surface, cytoplasmic or nuclear marker

88185 x 7-Flow cytometry; additional cell surface, cytoplasmic or nuclear marker (each)

88187-Flow Cytometry Interpretation, 2 to 8 markers

LOINC Code Information

Test ID Test Order Name Order LOINC Value
MONOF Monocyte Repartition by CD14/CD16,B 101146-9

 

Result ID Test Result Name Result LOINC Value
610019 % Monocytes of WBC 101147-7
610020 MO1 (Classical Monocytes) 101148-5
610023 Final Diagnosis In Process

Day(s) Performed

Monday through Saturday

Report Available

1 to 4 days