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Test Code MMLSA Antimicrobial Susceptibility, Anaerobic Bacteria, Minimal Inhibitory Concentration, Varies

Infectious

Reporting Name

Susceptibility, Anaerobic, MIC

Useful For

Determining the in vitro susceptibility on isolates of anaerobic bacteria involved in human infections

 

Directing antimicrobial therapy for anaerobic bacterial infections

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Varies


Shipping Instructions


For shipping information, see Infectious Specimen Shipping Guidelines.



Necessary Information


Organism identification and specimen source are required.



Specimen Required


Specimen Type: Organism in pure culture

Acceptable Sources: Isolates from blood cultures, bone and joint infections, or brain abscesses, and organisms isolated in pure culture from other sources

Supplies:

Anaerobic Transport Tube (T588)

Infectious Container, Large (T146)

Container/Tube:

Preferred: Anaerobic transport tube

Acceptable: Thioglycollate broth or any other suitable anaerobic transport system

Collection Instructions :

1. Organism must be in pure culture and actively growing. Do not submit mixed cultures.

2. Place specimen in a large infectious container and label as an etiologic agent/infectious substance if appropriate


Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Ambient

Reference Values

Susceptibility results are reported as minimal inhibitory concentration (MIC) in mcg/mL. Breakpoints (also known as clinical breakpoints) are used to categorize an organism as susceptible, susceptible-dose dependent, intermediate, resistant, or nonsusceptible according to breakpoint setting organizations, either the Clinical and Laboratory Standards Institute (CLSI) or the European Committee on Antimicrobial Susceptibility Testing (EUCAST), as applicable.

 

In some instances, an interpretive category cannot be provided based on available data; therefore, the following comment will be included on the report: There are no established interpretive guidelines for agents reported without interpretations.

 

For information regarding CLSI and EUCAST susceptibility interpretations, see Susceptibility Interpretative Category Definitions.

Day(s) Performed

Monday through Sunday

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

87186-Antimicrobial Susceptibility, Anaerobic Bacteria, MIC

87076-Organism Ref for ID, Anaerobic Bact (if appropriate)

87076-Anaerobe Ident (if appropriate)

87076-Id MALDI-TOF Mass Spec Anaerobe (if appropriate)

87153-Anaerobe ident by sequencing (if appropriate)

87150-Identification by PCR (if appropriate)

87185-Beta Lactamase (if appropriate)

87181-Anaerobe Susceptibility per Agent (if appropriate)

87150-mecA PCR (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
MMLSA Susceptibility, Anaerobic, MIC 50545-3

 

Result ID Test Result Name Result LOINC Value
MMLSA Susceptibility, Anaerobic, MIC 50545-3

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
ANAID Anaerobe Ident No, (Bill Only) No
RMALA Id MALDI-TOF Mass Spec Anaerobe No, (Bill Only) No
ISAN Anaerobe Ident by Sequencing No, (Bill Only) No
PCRID Identification by PCR No, (Bill Only) No
BLA Beta Lactamase No, (Bill Only) No
SANA Anaerobe Suscep per agent No, (Bill Only) No
ANIDE Organism Ref for ID, Anaerobic Bact Yes No
MECAB mecA PCR Test, Bill Only No, (Bill Only) No

Testing Algorithm

When this test is ordered, the reflex tests may be performed at an additional charge. All anaerobic bacterial organisms recovered will automatically have susceptibility testing performed and billed as appropriate. Antimicrobial agents appropriate to the organism and specimen source will be tested according to Mayo Clinic's practice and the laboratory's standard operating procedures.

 

For a listing of the antimicrobials routinely tested in this laboratory as well as antimicrobials that may be tested upon request, see Anaerobic Bacteria Antimicrobials. If the organism or antimicrobial agent of interest is not listed in this table, call 800-533-1710 and ask to speak to the Bacteriology Anaerobe Laboratory.

 

If organism identification is not provided within 72 hours of specimen receipt, referred anaerobic bacteria identification will be performed at an additional charge.

Interpretation

A "susceptible" category result and a low minimal inhibitory concentration value indicates in vitro susceptibility of the organism to the antimicrobial tested.

 

For interpretation of various antimicrobial susceptibility interpretive categories (ie, susceptible, intermediate, resistant, or epidemiological cutoff value), see Reference Values.

Clinical Reference

1. Rosenblatt JE, Brook I. Clinical relevance of susceptibility testing of anaerobic bacteria. Clin Infect Dis. 1993;16(Suppl 4):S446-S448

2. Jenkins SG, Schuetz AN. Current concepts in laboratory testing to guide antimicrobial therapy. Mayo Clin Proc. 2012;87(3):290-308

3. Schuetz AN, Carpenter DE. Susceptibility test methods: anaerobic bacteria. In: Carroll KC, Pfaller MA, eds. Manual of Clinical Microbiology. 12th ed. ASM Press; 2019:1377-1397

4. Jenkins SG, Schuetz AN. Current concepts in laboratory testing to guide antimicrobial therapy. Mayo Clin Proc. 2012;87(3):290-308

Report Available

8 to 14 days

Additional Tests

Test ID Reporting Name Available Separately Always Performed
BATTA Anaerobe Suscep Battery No Yes

Method Name

Minimal Inhibitory Concentration (MIC) by Agar Dilution

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.

Specimen Minimum Volume

See Specimen Required