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Test Code MHPRP Metamycoplasma hominis, Molecular Detection, PCR, Plasma

Useful For

Rapid, sensitive, and specific identification of Metamycoplasma hominis from plasma

 

This test is not intended for medicolegal use.

Method Name

Real-Time Polymerase Chain Reaction (PCR) using LightCycler and Fluorescent Resonance Energy Transfer (FRET)

Reporting Name

Mycoplasma hominis PCR, P

Specimen Type

Plasma EDTA


Ordering Guidance


 



Shipping Instructions


Ship specimen refrigerated.



Specimen Required


The high sensitivity of amplification by polymerase chain reaction requires the specimen to be processed in an environment in which contamination of the specimen by Metamycoplasma hominis DNA is unlikely.

 

Collection Container/Tube:

Preferred: Lavender top (EDTA)

Acceptable: Royal blue top (EDTA), pink top (EDTA)

Submission Container/Tube: Screw-capped, sterile container

Specimen Volume: 1 mL

Collection Instructions: Centrifuge and aliquot plasma into a sterile vial within 24 hours of collection.


Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Plasma EDTA Refrigerated (preferred) 7 days
  Frozen  7 days

Reference Values

Not applicable

Interpretation

A positive polymerase chain reaction (PCR) result for the presence of a specific sequence found within the Metamycoplasma hominis tuf gene indicates the presence of M hominis DNA in the specimen.

 

A negative PCR result indicates the absence of detectable M hominis DNA in the specimen, but it does not rule-out infection as false-negative results may occur due to inhibition of PCR, sequence variability underlying the primers and probes, or the presence of M hominis in quantities less than the limit of detection of the assay.

Clinical Reference

1. Sampath R, Patel R, Cunningham SA, et al: Cardiothoracic transplant recipient Mycoplasma hominis: An uncommon infection with probable donor transmission, EBioMedicine 2017 May;19:84-90

2. Waites KB, Taylor-Robinson D: Mycoplasma and Ureaplasma. In: Jorgensen JH, ed. Manual of Clinical Microbiology. 11th ed. ASM Press; 2015:1088-1105

Day(s) Performed

Monday through Friday

Report Available

3 to 4 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

87798

LOINC Code Information

Test ID Test Order Name Order LOINC Value
MHPRP Mycoplasma hominis PCR, P 68546-1

 

Result ID Test Result Name Result LOINC Value
MPSRC Specimen Source 31208-2
44134 Mycoplasma hominis PCR, P 68546-1