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HelpTest Code MHBRP Metamycoplasma hominis, Molecular Detection, PCR, Blood
Useful For
Rapid, sensitive, and specific identification of Metamycoplasma hominis from whole blood
This test is not intended for medicolegal use.
Method Name
Real-Time Polymerase Chain Reaction (PCR) using LightCycler and Fluorescent Resonance Energy Transfer (FRET)
Reporting Name
Mycoplasma hominis PCR, BSpecimen Type
Whole Blood EDTAShipping Instructions
Ship specimen refrigerated.
Specimen Required
The high sensitivity of amplification by polymerase chain reaction requires the specimen to be processed in an environment in which contamination of the specimen by Metamycoplasma hominis DNA is unlikely.
Container/Tube:
Preferred: Lavender top (EDTA)
Acceptable: Royal blue top (EDTA), pink top (EDTA), or sterile vial containing EDTA-derived aliquot
Specimen Volume: 1 mL
Collection Instructions: Send whole blood specimen in original tube (preferred). Do not aliquot.
Specimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Whole Blood EDTA | Refrigerated (preferred) | 7 days | |
| Frozen | 7 days |
Reference Values
Not applicable
Interpretation
A positive polymerase chain reaction (PCR) result for the presence of a specific sequence found within the Metamycoplasma hominis tuf gene indicates the presence of M hominis DNA in the specimen.
A negative PCR result indicates the absence of detectable M hominis DNA in the specimen, but it does not rule-out infection as falsely negative results may occur due to inhibition of PCR, sequence variability underlying the primers and probes, or the presence of M hominis in quantities less than the limit of detection of the assay.
Clinical Reference
1. Sampath R, Patel R, Cunningham SA, et al: Cardiothoracic transplant recipient Mycoplasma hominis: An uncommon infection with probable donor transmission, EBioMedicine. 2017 May;19:84-90. doi: 10.1016/j.ebiom.2017.04.026
2. Waites KB, Taylor-Robinson D: Mycoplasma and Ureaplasma. In: Jorgensen JH, ed. Manual of Clinical Microbiology. 11th ed. ASM Press; 2015:1088-1105
Day(s) Performed
Monday through Friday
Report Available
3 to 4 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
87798
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| MHBRP | Mycoplasma hominis PCR, B | 42621-3 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| MBSRC | Specimen Source | 31208-2 |
| 44131 | Mycoplasma hominis PCR, B | 42621-3 |