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HelpTest Code LYWB Lyme Disease Antibody, Immunoblot, Serum
Reporting Name
Lyme Disease Ab, Immunoblot, SUseful For
Aiding in the diagnosis of systemic Lyme disease
This test should not be used as a screening assay.
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
SerumSpecimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.75 mL Serum
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Specimen Minimum Volume
Serum: 0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Refrigerated (preferred) | 14 days |
| Frozen | 30 days |
Reference Values
IgG: Negative
IgM: Negative
Reference values apply to all ages
Day(s) Performed
Monday, Wednesday, Friday
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
86617 x 2
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| LYWB | Lyme Disease Ab, Immunoblot, S | 18203-0 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 5744 | IgG Immunoblot | 6320-6 |
| 2992 | IgG detected against: | 13502-0 |
| 23931 | IgM Immunoblot | 6321-4 |
| 23932 | IgM detected against: | 13503-8 |
| 6241 | Interpretation | 12781-1 |
Testing Algorithm
For information see Acute Tick-Borne Disease Testing Algorithm.
Interpretation
Positive:
The Lyme IgG Immunoblot is interpreted as positive if IgG-class antibodies are detected to greater than or equal to 5 Borrelia burgdorferi proteins.
The Lyme IgM Immunoblot is interpreted as positive if IgM-class antibodies are detected to greater than or equal to 2 B burgdorferi proteins.
Negative:
Immunoblot patterns not meeting the above criteria are interpreted as negative.
|
Result |
Interpretation |
|
IgG and IgM negative |
Specific serologic response to B burgdorferi infection is not detected but cannot rule out early infection during which low or undetectable antibody levels to B burgdorferi may be present. If clinically indicated, a new serum specimen should be submitted in 7 to 14 days. |
|
IgG positive and IgM negative |
Consistent with infection with B burgdorferi at some time in the past |
|
IgG and IgM positive |
Consistent with active or previous infection for B burgdorferi. IgM blot criteria is of diagnostic utility only during the first 4 weeks of early Lyme disease. |
|
IgG negative and IgM positive |
IgM-class antibodies to B burgdorferi (Lyme disease) detected. Results are consistent with acute or recent infection with B burgdorferi. Testing of a new specimen collected in 7 to 14 days to demonstrate IgG seroconversion may be considered to confirm infection if the diagnosis is in doubt. IgM immunoblot results should only be considered as indicative of recent infection in patients presenting within 30 days of symptom onset. Consideration of IgM immunoblot results in patients with symptoms lasting greater than 30 days is discouraged due to the risk of false positive IgM immunoblot results and/or prolonged IgM seropositivity following disease resolution. |
|
IgG and/or IgM uninterpretable |
Immunoblot invalid due to blurring or indistinct reactivity. Due to an invalid Lyme IgG/IgM immunoblot, an interpretation cannot be provided. Please submit a new specimen. |
Clinical Reference
Branda JA, Steere AC. Laboratory Diagnosis of Lyme Borreliosis. Clin Microbiol Rev. 2021;34(2):e00018-19. Published 2021 Jan 27. doi:10.1128/CMR.00018-19
Report Available
Same day/1 to 4 daysMethod Name
Immunoblot Microarray
Forms
If not ordering electronically, complete, print, and send 1 of the following with the specimen.
-Infectious Disease Serology Test Request (T916)
-General Test Request (T239)