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HelpTest Code LY27B HLA-B27, Blood
Reporting Name
HLA-B27, BUseful For
Assisting in the diagnostic process of ankylosing spondylitis, juvenile rheumatoid arthritis, and reactive arthritis
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
Whole Blood EDTAOrdering Guidance
This test is best used alone if a particular disease, such as ankylosing spondylitis, is under consideration.
Orders received for both this test and 1LRR / Human Leukocyte Antigens (HLA) Class I Typing Low Resolution, Recipient, Blood or 1DIS / Human Leukocyte Antigens (HLA) A-B-C Disease Association Typing Low Resolution, Blood (which provides data on all HLA Class I low-resolution antigens, including B27) will be questioned due to test overlap.
Shipping Instructions
Specimen must arrive within 96 hours of collection.
Specimen Required
Container/Tube: Lavender top (EDTA)
Specimen Volume: 6 mL
Collection Instructions: Send whole blood specimen in original tube. Do not aliquot.
Specimen Minimum Volume
1 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Whole Blood EDTA | Ambient | 4 days |
Reference Values
An interpretive report will be provided.
Day(s) Performed
Monday through Saturday
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
86812
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| LY27B | HLA-B27, B | 26028-1 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| LY27 | HLA-B27 Result | 26028-1 |
| B27C | Interpretation | 96625-9 |
Interpretation
Approximately 8% of the normal population carries the human leukocyte antigen (HLA)-B27.
HLA-B27 is present in approximately 89% of patients with ankylosing spondylitis, 79% of patients with reactive arthritis, and 42% of patients with juvenile rheumatoid arthritis. However, lacking other data, it is not diagnostic for these disorders.
Clinical Reference
1. Profaizer T, Dibb K, Bethers H, et al. Comparison of next-generation sequencing-based human leukocyte antigen typing with clinical flow cytometry and allele-specific PCR melting assays for HLA-B27 genotyping. J Appl Lab Med. 2021;6(5):1221-1227. doi:10.1093/jalm/jfab046
2. Skalska U, Kozakiewicz A, Maslinski W, Jurkowska M. HLA-B27 detection - comparison of genetic sequence-based method and flow cytometry assay. Reumatologia. 2015;53(2):74-78. doi:10.5114/reum.2015.51506
3. Brewerton DA, Hart FD, Nicholls A, Caffrey M, James DC, Sturrock RD. Ankylosing spondylitis and HL-A27. Lancet. 1973;1(7809):904-907
4. Albrecht J, Muller HA. HLA-B27 typing by use of flow cytofluorometry. Clin Chem. 1987;33(9):1619-1623
Report Available
2 to 6 daysMethod Name
Flow Cytometry
Forms
If not ordering electronically, complete, print, and send a General Request (T239) with the specimen.