Home
HelpTest Code LEPDT Leptospira, IgM, Serum
Useful For
Aiding in the diagnosis of leptospirosis
This test is not useful for establishing cure or response to therapy.
Method Name
Enzyme-Linked Immunoassay Dot (Immunodot)
Reporting Name
Leptospira, IgM, SSpecimen Type
SerumSpecimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.3 mL
Collection Instructions: Centrifuge and aliquot serum into plastic vial.
Additional Information: If acute and convalescent specimens are being obtained to determine seroconversion, they should be collected 2 or more weeks apart.
Specimen Minimum Volume
0.1 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Serum | Refrigerated (preferred) | 30 days | |
| Frozen | 30 days |
Reference Values
Negative
Interpretation
Positive:
IgM antibodies to Leptospira species detected, suggesting recent infection. Antibody presence alone cannot be used to definitively diagnose acute infection, as antibodies from a prior exposure or infection may remain detectable for a prolonged period.
Borderline:
Result should be interpreted with caution. Additional testing of a second, convalescent specimen is recommended. If the specimen remains borderline reactive, a second serological method should be considered if leptospirosis infection is still suspected.
Negative:
No IgM antibodies to Leptospira detected. Since antibodies may not be present or may be present at undetectable levels during early disease, repeat testing of a convalescent sample collected in 2 to 3 weeks is recommended.
Clinical Reference
1. Centers for Disease Control and Prevention, National Center for Emerging and Zoonotic Infectious Diseases (NCEZID), Division of High-Consequence Pathogens and Pathology (DHCPP): Leptospriosis. Reviewed June 24, 2024. Accessed September 5, 2024. Available at www.cdc.gov/leptospirosis/
2. Costa F, Hagan JE, Calcagno J, et al. Global morbidity and mortality of leptospirosis: a systemic review. PLoS Negl Trop Dis. 2015;9(9):e0003898. doi:10.1371/journal.pntd.0003898
Day(s) Performed
Monday, Thursday
Report Available
Same day/1 to 5 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
86720
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| LEPDT | Leptospira, IgM, S | 23201-7 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 65183 | Leptospira, IgM, S | 23201-7 |
Forms
If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.