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HelpTest Code LAGGN Granulocyte Antibody Screen, Serum
Specimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube: Red top (serum gel/SST are not acceptable)
Submission Container/Tube: Plastic vial
Specimen Volume: 1.5 mL
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Additional Information: Only a specimen collected before a transfusion reaction is acceptable.
Useful For
Work-up of individuals with autoimmune neutropenia
Work-up of individuals having febrile nonhemolytic transfusion reactions
Work-up for alloimmune neonatal neutropenia
This test is not useful for the diagnosis of neutropenia due to marrow suppression by drugs or tumors.
Method Name
Flow Cytometry/Agglutination
Reporting Name
Granulocyte Ab Screen, SSpecimen Type
Serum RedSpecimen Minimum Volume
0.3 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum Red | Refrigerated (preferred) | 30 days |
| Frozen | 365 days | |
| Ambient | 7 days |
Reference Values
Not applicable
Interpretation
A positive result can be due to anti-granulocyte antibodies and anti-human leukocyte antigen antibodies.
This test cannot distinguish between allo- or autoantibodies, nor can it determine the specificity of the detected antibody. Results should be correlated to clinical history.
Clinical Reference
1. Flesch BK, Reil A. Molecular genetics of the human neutrophil antigens. Transfus Med Hemother. 2018;45(5):300-309. doi:10.1159/000491031
2. Gottschall JL, Triulzi DJ, Curtis B, et al. The frequency and specificity of human neutrophil antigen antibodies in a blood donor population. Transfusion. 2011;51(4):820-827. doi:10.1111/j.1537-2995.2010.02913.x
3. Browne T, Dearman RJ, Poles A. Human neutrophil antigens: Nature, clinical significance and detection. Int J Immunogenet. 2021;48(2):145-156. doi:10.1111/iji.12514
4. Baig NA, Dukek BA, Falbo DK, et al. Daratumumab interference in flow cytometric anti-granulocyte antibody testing can be overcome using non-human blocking antibodies. Vox Sang. 2021;116(1):116-122. doi:10.1111/vox.12989
Day(s) Performed
Tuesday, Thursday
Report Available
7 to 15 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
86021 x2
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| LAGGN | Granulocyte Ab Screen, S | 105285-1 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| LAGG2 | GIFT/GAT Interpretation | 105288-5 |
| LAGG3 | GIFT Result | 105286-9 |
| LAGG4 | GAT Result | 105287-7 |