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Test Code KRASW KRAS Somatic Mutation Analysis, Peritoneal Fluid


Shipping Instructions


Send specimens refrigerated (4° C)



Necessary Information


1. A pathology report (final or preliminary) is required and must accompany specimen for testing to be performed.

2. The following information must be included in the report provided.

-Patient name and second identifier

-Date of fluid collection

-Source of the fluid



Specimen Required


Container/Tube: 50-mL Falcon tube

Preferred: Fresh, peritoneal washing; no fixatives added to wash

Specimen Volume: Two 50-mL Falcon tubes

Collection Instructions: Containers must be labeled with two unique patient identifiers.


Useful For

Staging of the pancreatic ductal adenocarcinoma(1)

Method Name

Droplet Digital Polymerase Chain Reaction (ddPCR)

Reporting Name

KRAS Mutation Analysis, Peritoneal

Specimen Type

Varies

Specimen Minimum Volume

100 mL of peritoneal washing

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Refrigerated

Reference Values

An interpretive report will be provided.

Interpretation

The interpretation of molecular biomarker analysis includes an overview of the results and the associated diagnostic, prognostic, and therapeutic implications.

Clinical Reference

1. Yonkus JA, Alva-Ruiz R, Abdelrahman AM, et al. Molecular peritoneal staging for pancreatic ductal adenocarcinoma using mutant KRAS droplet-digital polymerase chain reaction: Results of a prospective clinical trial. J Am Coll Surg. 2021;233(1):73-80.e1. doi:10.1016/j.jamcollsurg.2021.05.009

2. Kim NH, Kim HJ. Preoperative risk factors for early recurrence in patients with resectable pancreatic ductal adenocarcinoma after curative intent surgical resection. Hepatobiliary Pancreat Dis Int. 2018;17(5):450-455 doi:10.1016/j.hbpd.2018.09.003

3. Avula LR, Hagerty B, Alewine C. Molecular mediators of peritoneal metastasis in pancreatic cancer. Cancer Metastasis Rev. 2020;39(4):1223-1243. doi:10.1007/s10555-020-09924-4

Day(s) Performed

Monday through Friday

Report Available

5 to 10 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

81275-KRAS (v-Ki-ras2 Kirsten rat sarcoma viral oncogene) (eg, carcinoma) gene analysis, variants in codons 12 and 13

81276-KRAS (v-Ki-ras2 Kirsten rat sarcoma viral oncogene) (eg, carcinoma) gene analysis, additional variants

LOINC Code Information

Test ID Test Order Name Order LOINC Value
KRASW KRAS Mutation Analysis, Peritoneal 21702-6

 

Result ID Test Result Name Result LOINC Value
616453 Result Summary 50397-9
616454 Result 82939-0
616455 Interpretation 69047-9
616456 Specimen 31208-2
616457 Source 31208-2
616459 Released By 18771-6
616460 Method 85069-3
616461 Disclaimer 62364-5
616462 Additional Information 48767-8