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HelpTest Code JCV JC Virus Detection by In Situ Hybridization
Shipping Instructions
Attach the green "Attention Pathology" address label (T498) and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.
Necessary Information
A pathology or diagnostic report (final or preliminary) and brief history are required and must accompany specimen for testing to be performed.
Specimen Required
Specimen Type: Tissue
Supplies: Pathology Packaging Kit (T554)
Submit:
Formalin-fixed, paraffin-embedded tissue block (preferred)
OR
4 Unstained glass, positively-charged slides with 5 (+ or -1)-micron formalin-fixed, paraffin-embedded tissue
Additional Information: Broken or cracked unstained slides will be rejected.
Useful For
Confirming a clinical and histopathologic diagnosis of progressive multifocal leukoencephalopathy
Method Name
In Situ Hybridization (ISH)
Reporting Name
JC Virus ISHSpecimen Type
SpecialSpecimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Special | Ambient (preferred) | |
| Refrigerated | ||
Interpretation
This test, when not accompanied by a pathology consultation request, will be answered as either positive or negative. If additional interpretation or analysis is needed, request PATHC / Pathology Consultation along with this test.
Clinical Reference
1. Fritzsche FR, Pianca S, Gaspert A, et al. Silver-enhanced in situ hybridization for detection of polyomavirus DNA in patients with BK virus nephropathy. Diagn Mol Pathol. 2011;20(2):105-110
2. Aksamit AJ, Mourrain P, Sever JL, Major EO. Progressive multifocal leukoencephalopathy: investigation of 3 cases using in situ hybridization with JC virus biotinylated DNA probe. Ann Neurol. 1985;18(4):490-496
3. Aksamit AJ. Nonradioactive in situ hybridization in progressive multifocal leukoencephalopathy. Mayo Clin Proc. 1993;68(9):899-910
4. Muaoz-Marmol AM, Mola G, Fernandez-Vasalo A, et al. JC virus early protein detection by immunohistochemistry in progressive multifocal leukoencephalopathy: a comparative study with in situ hybridization and polymerase chain reaction. J Neuropathol Exp Neurol. 2004;63(11):1124-1130
5. Vago L, Cinque P, Sala E, et al. JCV-DNA and BKV-DNA in the CNS tissue and CSF of AIDS patients and normal subjects. Study of 41 cases and review of the literature. J Acquir Immune Defic Syndr Hum Retrovirol. 1996;12(2):139-146
6. Chen H, Chen XZ, Waterboer T, et al. Viral infections and colorectal cancer: a systematic review of epidemiological studies. Int J Cancer. 2015:137(1)12-24
Day(s) Performed
Monday through Friday
Report Available
5 to 7 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
88365-Primary
88364-If additional ISH
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| JCV | JC Virus ISH | In Process |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 71214 | Interpretation | 50595-8 |
| 71215 | Participated in the Interpretation | No LOINC Needed |
| 71216 | Report electronically signed by | 19139-5 |
| 71218 | Material Received | 81178-6 |
| 71596 | Disclaimer | 62364-5 |
| 72115 | Case Number | 80398-1 |
Forms
If not ordering electronically, complete, print, and send an Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request (T763) with the specimen.