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HelpTest Code HYDCU Hydrocodone with Metabolite Confirmation, Random, Urine
Useful For
Detection and quantification of hydrocodone, norhydrocodone, and hydromorphone in urine
Reporting Name
Hydrocodone w/metabolite Conf, USpecimen Type
UrineOrdering Guidance
For situations where chain of custody is required, a Chain of Custody Kit (T282) is available. For chain-of-custody testing, order OPATX / Opiates Confirmation, Chain of Custody, Random, Urine.
Additional drug panels and specific requests are available; call 800-533-1710.
Additional Testing Requirements
If urine creatinine is required or adulteration of the specimen is suspected, order ADULT / Adulterants Survey, Random, Urine.
Specimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube: Plastic urine container
Submission Container/Tube: Plastic, 5-mL tube
Specimen Volume: 3 mL
Collection Instructions:
1. Collect a random urine specimen.
2. No preservative.
Additional Information:
1. No specimen substitutions.
2. STATS are not accepted for this test.
3. Submitting less than 1 mL will compromise our ability to perform all necessary testing.
Specimen Minimum Volume
2.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Urine | Refrigerated (preferred) | 28 days |
| Frozen | 28 days | |
| Ambient | 14 days |
Reference Values
Negative
Positive results are reported with a quantitative result.
Cutoff concentrations by-liquid chromatography tandem mass spectrometry:
Hydrocodone: 25 ng/mL
Norhydrocodone: 25 ng/mL
Hydromorphone: 25 ng/mL
Interpretation
This procedure reports the total urine concentration; this is the sum of the unconjugated and conjugated forms of the parent drug.
Clinical Reference
1. Gutstein HB, Akil H. Opioid analgesics. In: Brunton LL, Lazo JS, Parker KL, eds. The Pharmacological Basis of Therapeutics. 11th ed. Goodman and Gilman's: McGraw-Hill Companies, Inc. 2006 Available at www.accessmedicine.com/content.aspx?aID=940653
2. Baselt RC, ed: Disposition of Toxic Drugs and Chemical in Man. 9th ed. Biomedical Publications; 2011
3. Hackett LP, Dusci LJ, Ilett KF, Chiswell GM. Optimizing the hydrolysis of codeine and morphine glucuronides in urine. Ther Drug Monit. 2002;24(5):652-657. doi:10.1097/00007691-200210000-00012
4. Langman LJ, Bechtel LK, Holstege CP. Clinical toxicology. In: Rifai N, Chiu RWK, Young I, Burnham CAD, Wittwer CT, eds Tietz Textbook of Laboratory Medicine. 7th ed. Elsevier; 2023:chap 43
Day(s) Performed
Monday through Friday
Report Available
2 to 5 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
G0480
80361 (if appropriate for select payers)
Clinical Toxicology CPT Code Client Guidance
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| HYDCU | Hydrocodone w/metabolite Conf, U | 74760-0 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 62614 | Hydrocodone-by LC-MS/MS | 16252-9 |
| 41999 | Norhydrocodone-by LC-MS/MS | 61422-2 |
| 35966 | Hydromorphone-by LC-MS/MS | 16998-7 |
| 36026 | Hydrocodone Interpretation | 69050-3 |
Method Name
Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
Forms
If not ordering electronically, complete, print, and send a Therapeutics Test Request (T831) with the specimen.