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HelpTest Code HTLVC Human T-Cell Lymphotropic Virus Types 1 and 2 (HTLV-1/-2) Antibody Screen with Confirmation, Spinal Fluid
Ordering Guidance
This test is for cerebrospinal fluid specimens only. For serum specimens, order HTLVI / Human T-Cell Lymphotropic Virus Types I and II Antibody Screen with Confirmation, Serum.
Necessary Information
Date of collection is required.
Specimen Required
Collection Container/Tube: Sterile vial
Specimen Volume: 1 mL
Collection Instructions: Submit spinal fluid specimen from collection vial 1.
Useful For
Qualitative screening detection of human T-cell lymphotropic virus types 1 and 2 (HTLV-1/-2) specific antibodies with confirmation and differentiation between HTLV-1 and HTLV-2 infection
This test should not be used as a screening or confirmatory test for any specimen other than spinal fluid.
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| HTLLC | HTLV -1/-2 Ab Confirmation, CSF | Yes | No |
Testing Algorithm
If human T-cell lymphotropic virus types 1 and 2 (HTLV-1/-2) antibody is reactive, then HTLV-1/-2 antibody confirmation by line immunoassay will be performed at an additional charge.
Method Name
Enzyme Immunoassay (EIA)
Reporting Name
HTLV-1/-2 Ab Screen, CSFSpecimen Type
CSFSpecimen Minimum Volume
0.6 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| CSF | Frozen (preferred) | 30 days |
| Refrigerated | 14 days |
Reference Values
Negative
Interpretation
Negative screening results indicate the absence of both human T-cell lymphotropic virus types 1 and 2 (HTLV-1/-2)-specific IgG antibodies in spinal fluid.
A reactive screening test result is suggestive of infection with either HTLV-1 or HTLV-2. However, this result does not confirm infection (eg, low specificity), and it cannot differentiate between HTLV-1 and HTLV-2 infection.
Specimens with reactive screening test results will be tested automatically by the line immunoassay (LIA) confirmatory test. Positive LIA results provide confirmatory evidence of infection with HTLV-1 or HTLV-2.
A reactive screening result with a negative or indeterminate confirmatory test result suggests either a false-reactive screening test result or a seroconverting HTLV infection. Repeat testing in 1 to 2 months can clarify the final infection status. Persistently indeterminate confirmatory test results indicate absence of HTLV infection.
Clinical Reference
1. Yamano Y, Sato T. Clinical pathophysiology of human T-lymphotropic virus-type I-associated myelopathy/tropical spastic paraparesis. Front Microbiol. 2012;3:389
2. Gessain A, Mahieux R. Tropical spastic paraparesis and HTLV-I associated myelopathy: clinical, epidemiological, virological, and therapeutic aspects. Rev Neurol (Paris). 2012;168(3):257-269
3. Marrero Rolon RM, Yao JDC. Laboratory diagnosis of HTLV-1-associated myelopathy. Clin Microbiol Newsl. 2020;42(16):129-134
Day(s) Performed
Monday, Tuesday, Thursday, Friday
Report Available
1 to 4 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test has been modified from the manufacturer's instructions. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
86790
86689 (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| HTLVC | HTLV-1/-2 Ab Screen, CSF | 22361-0 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 604934 | HTLV-1/-2 Ab Screen, CSF | 22361-0 |
Forms
If not ordering electronically, complete, print, and send Infectious Disease Serology Test Request (T916) with the specimen.