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Test Code HRTVS Heartland Virus, RNA, Molecular Detection, PCR, Serum


Ordering Guidance


Patients with a history of symptoms for more than 1 week may be negative by molecular tests (ie, real-time polymerase chain reaction) and may require serologic testing, which is available through the Centers for Disease Control and Prevention.



Specimen Required


Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container: Sterile container

Specimen Volume: 0.5 mL

Collection Instructions:

1. Within 2 hours of collection centrifuge and aliquot serum into a sterile container.

2. Serum specimens not aliquoted from the serum gel collection tube into a sterile container will be rejected.


Useful For

Aiding in the diagnosis of central nervous system infection caused by Heartland virus using serum specimens

Testing Algorithm

For more information see Meningitis/Encephalitis Panel Algorithm.

Method Name

Real-Time Polymerase Chain Reaction (PCR)

Reporting Name

Heartland Virus, PCR, Serum

Specimen Type

Serum

Specimen Stability Information

Specimen Type Temperature Time Special Container
Serum Refrigerated (preferred) 14 days
  Frozen  14 days
  Ambient  24 hours

Reference Values

Negative

Interpretation

Positive:

The detection of Heartland virus (HRTV) nucleic acid in serum is consistent with acute-phase infection. HRTV nucleic acid may be detectable during the first week following the onset of symptoms.

 

Negative:

The absence of HRTV nucleic acid is consistent with the lack of acute-phase infection but does not rule out infection. Additional serologic testing may be indicated.

Clinical Reference

1. Savage HM, Godsey MS, Lambert A, et al. First detection of heartland virus (Bunyaviridae: Phlebovirus) from field collected arthropods. Am J Trop Med Hyg. 2013;89(3):445-452. doi:10.4269/ajtmh.13-0209

2. Brault AC, Savage HM, Duggal NK, Eisen RJ, Staples JE. Heartland virus epidemiology, vector association, and disease potential. Viruses. 2018;10(9):498. doi:10.3390/v10090498

3. Staples JE, Pastula DM, Panella AJ, et al. Investigation of Heartland virus disease throughout the United States, 2013–2017. Open Forum Infect Dis. 2020;7(5):ofaa125. Published 2020 Apr 11. doi:10.1093/ofid/ofaa125

Day(s) Performed

Monday through Sunday

Report Available

Same day/1 to 5 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

87798

LOINC Code Information

Test ID Test Order Name Order LOINC Value
HRTVS Heartland Virus, PCR, Serum 94183-1

 

Result ID Test Result Name Result LOINC Value
620057 Heartland Virus, PCR, Serum 94183-1

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.