Test Code HIAAP 5-Hydroxyindoleacetic Acid, Plasma
Ordering Guidance
This test quantifies 5-hydroxyindoleacetic acid in plasma specimens and is comparable to testing on 24-hour and random urine specimens for the diagnosis and monitoring of intestinal carcinoid syndrome.
Necessary Information
Patient's age is required.
Specimen Required
Patient Preparation:
1. Some medications could interfere with test results. If medically feasible, for 48 hours before and during specimen collection, patient should not take the following medications:
-Acetaminophen (Tylenol or generic versions)
-Tryptophan containing supplements.
2. For 24 hours prior to and during collection, the patient should limit the following to one serving per day:
-Fruits
-Vegetables
-Caffeinated beverages or foods
3. For 24 hours prior to and during collection, patients should not eat nuts, especially walnuts. Plasma 5-hydroxyindoleacetic acid levels revert to baseline levels when walnuts are ingested after other foods.
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube:
Preferred: Green top (sodium heparin)
Acceptable: Lavender top (EDTA)
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions:
1. Centrifuge at 4° C.
2. Aliquot plasma into plastic vial.
3. Send plasma frozen.
Useful For
Biochemical diagnosis and monitoring of intestinal carcinoid syndrome using a plasma specimen
Method Name
Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
Reporting Name
5-Hydroxyindoleacetic Acid, PSpecimen Type
PlasmaSpecimen Minimum Volume
0.2 mL
Specimen Stability Information
Specimen Type | Temperature | Time |
---|---|---|
Plasma | Frozen (preferred) | 60 days |
Refrigerated | 72 hours |
Reference Values
≤6 months: ≤130 ng/mL
>6 months: ≤30 ng/mL
Interpretation
If pharmacological and dietary artifacts have been ruled out, an elevated concentration of 5-hydroxyindoleacetic acid is a probable indicator of the presence of a serotonin-producing tumor.
Clinical Reference
1. Oleinikov K, Avniel-Polak S, Gross DJ, Grozinsky-Glasberg S. Carcinoid syndrome: Updates and review of current therapy. Curr Treat Options Oncol. 2019 9;20(9):70. doi:10.1007/s11864-019-0671-0
2. Adaway JE, Dobson R, Walsh J, et al. Serum and plasma 5-hydroxyindoleacetic acid as an alternative to 24-h urine 5-hydroxyindoleacetic acid measurement. Ann Clin Biochem. 2016;53(Pt 5):554-560. doi:10.1177/0004563215613109
3. Tohmola N, Johansson A, Sane T, Renkonen R, Hamalainen E, Itkonen O. Transient elevation of serum 5-HIAA by dietary serotonin and distribution of 5-HIAA in serum protein fractions. Ann Clin Biochem. 2015;52(Pt 4):428-433. doi:10.1177/0004563214554842
4. Tellez MR, Mamikunian G, O'Dorisio TM, Vinik AI, Woltering EA. A single fasting plasma 5-HIAA value correlates with 24-hour urinary 5-HIAA values and other biomarkers in midgut neuroendocrine tumors (NETs). Pancreas. 2013;42(3):405-410. doi:10.1097/MPA.0b013e318271c0d5
Day(s) Performed
Monday, Wednesday, Friday
Report Available
3 to 5 daysPerforming Laboratory

Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
83497
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
HIAAP | 5-Hydroxyindoleacetic Acid, P | 1693-1 |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
619735 | 5-Hydroxyindoleacetic Acid, P | 1693-1 |