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Test Code GFATC Glial Fibrillary Acidic Protein Alpha Subunit Antibody, Immunofluorescence Titer Assay, Spinal Fluid


Necessary Information


Provide the following information:       

-Relevant clinical information

-Ordering provider name, phone number, mailing address, and e-mail address



Specimen Required


Only orderable as a reflex. For more information, see:

DMC2 / Dementia Autoimmune Evaluation, Spinal Fluid

ENC2 / Encephalopathy Autoimmune Evaluation, Spinal Fluid

EPC2 / Epilepsy Autoimmune Evaluation, Spinal Fluid

MAC1 / Autoimmune Myelopathy Evaluation, Spinal Fluid

 

Container/Tube: Sterile vial

Specimen Volume: 2 mL


Useful For

Reporting an end titer result in spinal fluid specimens

 

Distinguishing, in spinal fluid, autoimmune GFAP astrocytopathy from infectious meningoencephalitis and idiopathic inflammatory central nervous system (CNS) disorders such as multiple sclerosis, vasculitis and sarcoidosis, disorders commonly considered in the differential diagnosis

 

Alerting the clinician that the patient has an immune-mediated, steroid-responsive disorder and to search for a malignancy

Testing Algorithm

If immunofluorescence assay (IFA) pattern suggests GFAP, then GFAP IFA titer and GFAP cell-binding assay (CBA) are performed at an additional charge.

Method Name

Only orderable as a reflex. For more information, see:

DMC2 / Dementia Autoimmune Evaluation, Spinal Fluid

ENC2 / Encephalopathy Autoimmune Evaluation, Spinal Fluid

EPC2 / Epilepsy Autoimmune Evaluation, Spinal Fluid

MAC1 / Autoimmune Myelopathy Evaluation, Spinal Fluid

 

Indirect Immunofluorescence Assay (IFA)

Reporting Name

GFAP IFA Titer, CSF

Specimen Type

CSF

Specimen Minimum Volume

1.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
CSF Refrigerated (preferred) 28 days
  Frozen  28 days
  Ambient  72 hours

Reference Values

Only orderable as a reflex. For more information, see:

DMC2 / Dementia Autoimmune Evaluation, Spinal Fluid

ENC2 / Encephalopathy Autoimmune Evaluation, Spinal Fluid

EPC2 / Epilepsy Autoimmune Evaluation, Spinal Fluid

MAC1 / Autoimmune Myelopathy Evaluation, Spinal Fluid

 

<1:2

Interpretation

Seropositivity for autoantibody (positive) is supportive of autoimmune glial fibrillary acidic protein (GFAP) astrocytopathy, a treatable form of meningoencephalomyelitis. A paraneoplastic basis should be considered, according to age, sex, and other risk factors.

 

Patients who are GFAP-IgG positive have increased risk of tumor. GFAP-IgG increases the likelihood of certain malignancies being found within 2 years of symptom onset (34%). The most common malignancy found is ovarian teratoma (22%).

 

GFAP meningoencephalomyelitis is immunotherapy-responsive. GFAP-IgG positive patients have better outcomes after treatment with corticosteroids.

 

The presence of GFAP-IgG alerts the clinician the patient has an immune-mediated, steroid-responsive disorder and directs patient care accordingly. It also alerts the clinician to search for a malignancy.

Clinical Reference

1. Fang B, McKeon A, Hinson SR, et al: Autoimmune glial fibrillary acidic protein astrocytopathy: a novel meningoencephalomyelitis. JAMA Neurol 2016;73:1297-1307

2. Flanagan EP, Hinson SR, Lennon VA, et al: Glial fibrillary acidic protein immunoglobulin G as biomarker of autoimmune astrocytopathy: Analysis of 102 patients. Ann Neurol 2017;81:298-309

3. Iorio R, Damato V, Evoli A, et al:Clinical and Immunological characteristics of the spectrum of GFAP autoimmunity: a case series of 22 patients. J Neurol Neurosurg Psychiatry 2018 Feb;89(2):138-146 doi:10.1136/jnnp-2017-316583

Day(s) Performed

Monday through Sunday

Report Available

10 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

86256

LOINC Code Information

Test ID Test Order Name Order LOINC Value
GFATC GFAP IFA Titer, CSF 93421-6

 

Result ID Test Result Name Result LOINC Value
605134 GFAP IFA Titer, CSF 93421-6