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Test Code F_12 Coagulation Factor XII Activity Assay, Plasma

Important Note

  • Must be drawn at Outpatient Lab at the Main Campus
  • Collect in Light Blue Tube

Reporting Name

Coag Factor XII Assay, P

Useful For

Diagnosing deficiency of coagulation factor XII

 

Determining cause of prolonged activated partial thromboplastin time

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Plasma Na Cit


Ordering Guidance


Coagulation testing is highly complex, often requiring the performance of multiple assays and correlation with clinical information. For that reason, consider ordering a Coagulation Consultation.



Necessary Information


If priority specimen, mark request form, give reason, and request a call-back.



Specimen Required


Specimen Type: Platelet-poor plasma

Patient Preparation: Patient must not be receiving Coumadin (warfarin) or heparin therapy.

Collection Container/Tube: Light-blue top (3.2% sodium citrate)

Submission Container/Tube: Plastic vial

Specimen Volume: 1 mL

Collection Instructions:

1. Specimen must be collected prior to factor replacement therapy

2. For complete instructions, see Coagulation Guidelines for Specimen Handling and Processing.

3. Centrifuge, transfer all plasma into a plastic vial, and centrifuge plasma again.

4. Aliquot plasma into a plastic vial, leaving 0.25 mL in the bottom of centrifuged vial.

5. Freeze plasma immediately (no longer than 4 hours after collection) at -20° C or ideally, at or below -40° C.

Additional Information:

1. Double-centrifuged specimen is critical for accurate results as platelet contamination may cause spurious results.

2. Each coagulation assay requested should have its own vial.


Specimen Minimum Volume

0.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Plasma Na Cit Frozen 14 days

Reference Values

Adults: 55-180%

Normal, full-term newborn infants or healthy premature infants may have decreased levels (≥15% to 20%), which may not reach adult levels for 180 or more days postnatal.*

*See Pediatric Hemostasis References section in Coagulation Guidelines for Specimen Handling and Processing.

Day(s) Performed

Monday through Saturday

Test Classification

This test has been modified from the manufacturer's instructions. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

85280

LOINC Code Information

Test ID Test Order Name Order LOINC Value
F_12 Coag Factor XII Assay, P 3232-6

 

Result ID Test Result Name Result LOINC Value
F_12 Coag Factor XII Assay, P 3232-6

Interpretation

Acquired deficiency is associated with liver disease, nephritic syndrome, and chronic granulocytic leukemia.

 

Congenital homozygous deficiency: 20% activity

 

Congenital heterozygous deficiency: 20% to 50% activity

Clinical Reference

1. Renne T, Schmaier AH, Nickel KF, Blomback M, Maas C. In vivo roles of factor XII. Blood. 2012;120(22):4296-4303

2. Favaloro EJ, Lippi G, eds. Hemostasis and Thrombosis: Methods and Protocols. Humana Press; 2017

Report Available

1 to 3 days

Method Name

Optical Clot-Based