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Test Code FSCOV SARS-CoV-2 (COVID-19) Qualitative Real -Time-PCR

Important Note

  • Must be ordered as  .Mayo Miscellaneous
  • Test Code: FSCOV
  • Test Name: SARS-CoV-2 (COVID-19) Qualitative Real-Time RT-PCR

Methodology

TH UW SARS-CoV-2 Real-time RT-PCR assay targets two distinct regions of SARS-CoV-2 (the causative agent for COVID-19), the RNA-dependent RNA polymerase gene and the E gene.

Amplification of both targets results in a presumptive positive test result, while amplification of one of the two targets results in an inconclusive result, and amplification of neither target results a negative result.

Samples with positive or inconclusive test results require further testing and will be redirected to CDC and/or state public health lab.

 

 

Specimen Requirements

Collect: Nasopharyngeal Swab

Specimen should be collected by a doctor, PA, NP, nurse or Respiratory Therapist

Collection instructions: Gently insert mini-tipped flocked nasopharyngeal swab (swab on flexible plastic shaft through the nostril and into the nasopharynx, reaching the posterior nasopharynx. Gently rotate swab, then remove, break swab off into universal transport media (UTM), or viral transport medium (VTM). Make sure swab is fully immersed and that the shaft is short enough to completely tighten the cap. Send swab refrigerated immediately.

Sample may be stored at 2-8°C for up to 72 hours of collection.

Ship immediately to lab at 2-8°C (ice pack).

If collection to receipt in the lab will exceed 72 hours freeze at -10°C or colder and ship on dry ice.

*Do Not freeze/thaw"

Note: M5 and M6 are not acceptable for testing

Days and Set-up

  • Multiple times a day
  • Min/Max Time 2-5 days

Specimen Stability

72 hours refrigerated

CPT Code

U0002