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HelpTest Code DLL3 Delta-Like 3 Protein (SP347), Semi-Quantitative Immunohistochemistry, Manual, Tissue
Shipping Instructions
Attach the green "Attention Pathology" address label (T498) and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.
Necessary Information
A pathology/diagnostic report and a brief history are required.
Specimen Required
Specimen Type: Tissue
Supplies:
-Pathology Packaging Kit (T554)
-Immunostain Technical Only Envelope (T693)
Container/Tube: Immunostain Technical Only Envelope
Collection Instructions: Formalin-fixed, paraffin-embedded tissue block; or 3 unstained glass, “positively charged†slides with 4-microns, formalin-fixed, paraffin-embedded tissue
Additional Information: One slide will be stained with hematoxylin and eosin and returned.
Useful For
Diagnosis of small cell lung carcinoma (SCLC), large cell neuroendocrine carcinoma (LCNEC), amongst other tumors
Method Name
Immunohistochemistry (IHC)
Reporting Name
DLL3(SP347)Semi-Quant IHC,ManualSpecimen Type
SpecialSpecimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Special | Ambient (preferred) | ||
| Refrigerated | |||
Interpretation
Positivity for this test will require 50% or more of the tumor cells within a specimen to express delta-like 3 protein with an intensity ranging from 1 to 3+.
Clinical Reference
1. Saunders LR, Bankovich AJ, Anderson WC, et al. A DLL3-targeted antibody-drug conjugate eradicates high-grade pulmonary neuroendocrine tumor-initiating cells in vivo. Sci Transl Med. 2015;7(302):302ra136
2. Xie H, Boland JM, Maleszewski JJ, et al. Expression of delta-like protein 3 is reproducibly present in a subset of small cell lung carcinomas and pulmonary carcinoid tumors. Lung Cancer. 2019;135:73-79. doi:10.1016/j.lungcan.2019.07.016
3. Xie H, Kaye FJ, Isse K, et al. Delta-like protein 3 expression and targeting in merkel cell carcinoma. Oncologist. 2020;10:810-817. doi:10.1634/theoncologist.2019-0877
4. Magaki S, Hojat SA, Wei B, So A, Yong WH. An introduction to the performance of immunohistochemistry. Methods Mol Biol. 2019;1897:289-298. doi:10.1007/978-1-4939-8935-5_25
Day(s) Performed
Monday through Friday
Report Available
5 to 7 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
88360
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| DLL3 | DLL3(SP347)Semi-Quant IHC,Manual | In Process |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 603333 | Interpretation | 59465-5 |
| 603334 | Participated in the Interpretation | No LOINC Needed |
| 603335 | Report electronically signed by | 19139-5 |
| 603336 | Material Received | 81178-6 |
| 603337 | Disclaimer | 62364-5 |
| 603338 | Case Number | 80398-1 |
Forms
If not ordering electronically, complete, print, and send a Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request (T763) with the specimen.