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Test Code CYTH2 T-Helper Cell Type 2 Cytokine Panel, Plasma


Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube: Lavender top (EDTA)

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL

Collection Instructions:

1. Immediately after specimen collection, place the tube on wet ice.

2. Within 2 hours of collection, centrifuge at 4° C, 1500 x g for 10 minutes and aliquot plasma into a plastic vial.

3. Freeze immediately.

Additional Information:

1. Plasma for this test cannot be shared with other tests. Submit specimen for this test in its own vial.

2. This test cannot be added onto a previously collected specimen.


Useful For

Assessment of plasma cytokine levels to understand the etiology of allergic conditions and parasitic infections when used in conjunction with clinical information and other laboratory testing

Method Name

Bead-Based Multiplex Immunoassay

Reporting Name

Th2 Cytokine Panel, P

Specimen Type

Plasma EDTA

Specimen Minimum Volume

0.3 mL

Specimen Stability Information

Specimen Type Temperature Time
Plasma EDTA Frozen 21 days

Reference Values

IL-4:

<18 years: Not established

≥18 years: <100 pg/mL

 

IL-5:

<18 years: Not established

≥18 years: <20.0 pg/mL

 

IL-10:

<18 years: Not established

≥18 years: <7.0 pg/mL

 

IL-13:

<18 years: Not established

≥18 years: <700 pg/mL

Interpretation

Elevated cytokine concentrations could be consistent with the presence of allergic conditions or parasitic infections.

Clinical Reference

1. Cousins DJ, Lee TH, Staynov DZ. Cytokine coexpression during human Th1/Th2 cell differentiation: direct evidence for coordinated expression of Th2 cytokines. J Immunol. 2002;169(5):2498-2506

2. Junttila IS. Tuning the Cytokine Responses: An Update on Interleukin (IL)-4 and IL-13 Receptor Complexes. Front Immunol. 2018;9:888. doi:10.3389/fimmu.2018.00888

3. Jackson DJ, Wechsler ME, Brusselle G, Buhl R. Targeting the IL-5 pathway in eosinophilic asthma: A comparison of anti-IL-5 versus anti-IL-5 receptor agents. Allergy. 2024;79(11):2943-2952

4. Simpson EL, Guttman-Yassky E, Eichenfield LF, et al. Tralokinumab therapy for moderate-to-severe atopic dermatitis: Clinical outcomes with targeted IL-13 inhibition. Allergy. 2023;78(11):2875-2891

5. Hirahara K, Aoki A, Nakayama T. Pathogenic helper T cells. Allergol Int. 2021;70(2):169-173. doi:10.1016/j.alit.2021.02.001

6. Li KP, Shanmuganad S, Carroll K, Katz JD, Jordan MB, Hildeman DA. Dying to protect: cell death and the control of T-cell homeostasis. Immunol Rev. 2017;277(1):21-43. doi:10.1111/imr.12538

Day(s) Performed

Wednesday

Report Available

2 to 8 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

83520 x4

LOINC Code Information

Test ID Test Order Name Order LOINC Value
CYTH2 Th2 Cytokine Panel, P 82335-1

 

Result ID Test Result Name Result LOINC Value
622931 IL-4 27161-9
622933 IL-5 33938-2
622930 IL-10 26848-2
622932 IL-13 33822-8