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HelpTest Code CTBID Culture Referred for Identification, Mycobacterium and Nocardia, Varies
Infectious
Reporting Name
Culture Refer for ID, MycobacteriumUseful For
Rapid identification to the species level for Mycobacterium species, Nocardia species, and other aerobic actinomycete genera and species from pure culture isolates
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
VariesShipping Instructions
1. See Infectious Specimen Shipping Guidelines.
2. Place specimen in a large infectious container (T146) and label as an etiologic agent/infectious substance.
Necessary Information
1. Specimen source is required.
2. Isolate description is required: Gram stain reaction, morphology, tests performed.
Specimen Required
Specimen Type: Mycobacterium species or aerobic actinomycetes organism in pure culture
Supplies: Infectious Container, Large (T146)
Container/Tube: Middlebrook (7H10 or 7H11) or Lowenstein-Jensen medium slant or in broth (eg, Mycobacteria Growth Indicator Tube [7H9] broth)
Specimen Volume:
Solid media: Visible growth of isolate
Isolate in broth media: ≥3 mL
Collection Instructions: Organism must be in pure culture, actively growing. Do not submit mixed cultures.
Additional Information: A minimum volume of 3 mL is recommended to perform all initial testing, this may include: stains, sub-culture media, nucleic acid probes, and any additional testing that may be required to determine the identification. If the broth sample volume is less than 3 mL, initial testing may be limited, and increased turnaround time is likely.
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Varies | Ambient (preferred) | |
| Refrigerated | ||
Special Instructions
Reference Values
Not applicable
Day(s) Performed
Monday through Sunday
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| CTBID | Culture Refer for ID, Mycobacterium | 543-9 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| CTBID | Culture Refer for ID, Mycobacterium | In Process |
Testing Algorithm
Reflex tests may be performed at an additional charge. All mycobacteria and Nocardia (including aerobic actinomycetes) submitted will be identified and billed as appropriate.
See Culture Referred for Identification Mycobacterium and Nocardia Algorithm.
Interpretation
Organisms growing in pure culture are identified to the species level where indicated.
Clinical Reference
1. Martin I, Pfyffer GE, Parrish N. Mycobacterium: General characteristics, laboratory detection and staining procedures. In: Carroll KC, Pfaller MA, Landry ML, et al, eds. Manual of Clinical Microbiology. 12th ed. Vol 1. ASM Press; 2019:558-575
2. Warshauer DM, Salfinger M, Desmond E, Lin SYG. Mycobacterium tuberculosis complex. In: Carroll KC, Pfaller MA, Landry ML, et al, eds. Manual of Clinical Microbiology. 12th ed. Vol 1. ASM Press; 2019:576-594
3. Caulfield AJ, Richter E, Brown-Elliott BA, Wallace RJ Jr, Wengenack NL. Mycobacterium: Laboratory characteristics of slowly growing mycobacteria other than Mycobacterium tuberculosis. In: Carroll KC, Pfaller MA, Landry ML, et al, eds. Manual of Clinical Microbiology. 12th ed. Vol 1. ASM Press; 2019:595-611
4. Brown-Elliott BA, Wallace RJ Jr. Mycobacterium: Clinical and laboratory characteristics of rapidly growing mycobacteria. In: Carroll KC, Pfaller MA, Landry ML, et al, eds. Manual of Clinical Microbiology. 12th ed. Vol 1. ASM Press; 2019:612-629
5. Conville PS, Brown-Elliott BA, Witebsky FG. Nocarida, rhodococcus, gordonia, actinomadura, streptomyces and other aerobic actinomycetes. In: Carroll KC, Pfaller MA, Landry ML, et al, eds. Manual of Clinical Microbiology. 12th ed. Vol 1. ASM Press; 2019:525-557
Report Available
60 to 70 daysReflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| RMALM | Id MALDI-TOF Mass Spec AFB | No, (Bill Only) | No |
| RTBSP | Id, Mtb Speciation, PCR | No, (Bill Only) | No |
| ISMY | ID by 16S Sequencing | No, (Bill Only) | No |
| LCTB | Id, MTB complex Rapid PCR | No, (Bill Only) | No |
CPT Code Information
Culture Referred for Identification, Mycobacterium
87118-Identification of mycobacteria
87158-Identification of mycobacteria by other methods (if appropriate)
87118 -Id MALDI-TOF Mass Spec AFB (if appropriate)
87153-Mycobacteria Identification by Sequencing (if appropriate)
87150-Id, Mtb Speciation, PCR (if appropriate)
87150- Id, MTB complex Rapid PCR (if appropriate)
Method Name
Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS)/16S rDNA Sequencing/Rapid Polymerase Chain Reaction (PCR)
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.
Specimen Minimum Volume
See Specimen Required