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Test Code CRTHT Double-Stranded DNA (dsDNA) IgG Antibodies, Crithidia luciliae Immunofluorescence Titer, Serum (Non-Orderable)


Specimen Required


Only orderable as a reflex. For more information see CRTHS / Double-Stranded DNA (dsDNA) IgG Antibodies, Crithidia luciliae Immunofluorescence Screen, Serum.

 

Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube:

Preferred: Serum gel

Acceptable: Red top

Submission Container/Tube: Plastic vial

Specimen Volume: 0.8 mL Serum

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.


Useful For

Only orderable as a reflex. For more information see CRTHS / Double-Stranded DNA (dsDNA) IgG Antibodies, Crithidia luciliae Immunofluorescence Screen, Serum.

 

Antibody titer testing for antibodies to double-stranded DNA using the Crithidia luciliae indirect immunofluorescence test is part of the evaluation of patients with clinical features of systemic lupus erythematosus (SLE) or at-risk for disease

 

This test should not be used independently for the diagnosis and management of patients with SLE.

Method Name

Only orderable as a reflex. For more information see CRTHS / Double-Stranded DNA (dsDNA) IgG Antibodies, Crithidia luciliae Immunofluorescence Screen, Serum.

 

Indirect Immunofluorescence Assay (IFA)

Reporting Name

dsDNA Ab IFA Titer, IgG, S

Specimen Type

Serum

Specimen Minimum Volume

See Specimen Required

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 7 days
  Frozen  21 days

Reference Values

Only orderable as a reflex. For more information see CRTHS / Double-Stranded DNA (dsDNA) IgG Antibodies, Crithidia luciliae Immunofluorescence Screen, Serum.

 

Negative (<1:10)

Interpretation

The presence of IgG antibodies to double-stranded DNA detected using the Crithidia luciliae indirect immunofluorescence test is highly specific for systemic lupus erythematosus (SLE) with moderate sensitivity.

 

A negative result does not exclude a diagnosis of SLE.

Clinical Reference

1. Petri M, Orbai AM, Alarcon GS, et al. Derivation and validation of the Systemic Lupus International Collaborating Clinics classification criteria for systemic lupus erythematosus. Arthritis Rheum. 2012;64(8): 2677-2686

2. Aringer M, Costenbader K, Daikh D, et al. 2019 European League Against Rheumatism/American College of Rheumatology Classification Criteria for systemic lupus erythematosus. Arthritis Rheumatol. 2019;71(9):1400-1412

3.Orme ME, Voreck A, Aksouh R, Schreurs MWJ. Anti-dsDNA testing specificity for systemic lupus erythematosus: A systematic review. J Appl Lab Med. 2022;7(1):221-239. doi:10.1093/jalm/jfab146

4. Enocsson H, Sjowall C, Wirestam L, et al. Four anti-dsDNA antibody assays in relation to systemic lupus erythematosus disease specificity and activity. J Rheumatol. 2015;42(5):817-825

5. Sarbu MI, Salman-Monte TC, Rubio Munoz P, Lisbona MP, Almirall Bernabe M, Carbonell J. Differences between clinical and laboratory findings in patients with recent diagnosis of SLE according to the positivity of anti-dsDNA by the Crithidia luciliae method. Lupus. 2015;24(11):1198-1203

6. Bragazzi NL, Watad A, Damiani G, Adawi M, Amital H, Shoenfeld Y. Role of anti-DNA auto-antibodies as biomarkers of response to treatment in systemic lupus erythematosus patients: hypes and hopes. Insights and implications from a comprehensive review of the literature. Expert Rev Mol Diagn. 2019;19(11):969-978

7. Zaminski D, Saxena A, Izmirly P, Buyon JP, Belmont HM. Clinical implications of discordance between anti-dsDNA antibodies by multiplex flow immunoassay and Crithidia luciliae assay in a multiethnic racial cohort of patients with SLE. Lupus Sci Med. 2023;10(2):e001012

8. Cockx M, Van Hoovels L, De Langhe E, et al. Laboratory evaluation of anti-dsDNA antibodies. Clin Chim Acta. 2022;528:34-43

Day(s) Performed

Monday through Friday

Report Available

2 to 4 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

86256

LOINC Code Information

Test ID Test Order Name Order LOINC Value
CRTHT dsDNA Ab IFA Titer, IgG, S 58466-4

 

Result ID Test Result Name Result LOINC Value
623427 dsDNA Ab IFA Titer, IgG, S 58466-4