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HelpTest Code CRTHS Double-Stranded DNA (dsDNA) IgG Antibodies, Crithidia luciliae Immunofluorescence Screen, Serum
Specimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.7 mL Serum
Collection Instructions: Centrifuge and aliquot serum into a plastic vial.
Useful For
Evaluation and management of anti-double-stranded DNA IgG antibodies in patients with clinical features of systemic lupus erythematosus (SLE) or at-risk for disease
This test should not be used independently for the diagnosis and management of patients with SLE.
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| CRTHT | dsDNA Ab IFA Titer, IgG, S | No | No |
Testing Algorithm
If the double-stranded DNA (dsDNA) IgG antibodies Crithidia luciliae immunofluorescence screen is positive, then the dsDNA IgG antibodies Crithidia luciliae immunofluorescence titer will be performed at an additional charge.
Method Name
CRTHS, CRTHT: Indirect Immunofluorescence Assay (IFA)
Reporting Name
dsDNA Ab IFA Screen, IgG, SSpecimen Type
SerumSpecimen Minimum Volume
See Specimen Required
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum | Refrigerated (preferred) | 7 days |
| Frozen | 21 days |
Reference Values
DOUBLE-STRANDED DNA (dsDNA) IgG ANTIBODIES, CRITHIDIA LUCILIAE IMMUNOFLUORESCENCE SCREEN
Negative (<1:10)
Reference values apply to all ages.
DOUBLE-STRANDED DNA (dsDNA) IgG ANTIBODIES, CRITHIDIA LUCILIAE IMMUNOFLUORESCENCE TITER
Negative (<1:10)
Interpretation
The presence of IgG antibodies to double-stranded DNA detected using the Crithidia luciliae indirect immunofluorescence test is highly specific for systemic lupus erythematosus (SLE) with moderate sensitivity.
A negative result does not exclude a diagnosis of SLE.
Clinical Reference
1. Petri M, Orbai AM, Alarcon GS, et al. Derivation and validation of the Systemic Lupus International Collaborating Clinics classification criteria for systemic lupus erythematosus. Arthritis Rheum. 2012;64(8):2677-2686
2. Aringer M, Costenbader K, Daikh D, et al. 2019 European League Against Rheumatism/American College of Rheumatology Classification Criteria for systemic lupus erythematosus. Arthritis Rheumatol. 2019;71(9):1400-1412
3. Orme ME, Voreck A, Aksouh R, Schreurs MWJ. Anti-dsDNA Testing Specificity for Systemic Lupus Erythematosus: A systematic review. J Appl Lab Med. 2022;7(1):221-239. doi:10.1093/jalm/jfab146
4. Enocsson H, Sjowall C, Wirestam L, et al. Four anti-dsDNA antibody assays in relation to systemic lupus erythematosus disease specificity and activity. J Rheumatol. 2015;42(5):817-825
5. Sarbu MI, Salman-Monte TC, Rubio Munoz P, Lisbona MP, Almirall Bernabe M, Carbonell J. Differences between clinical and laboratory findings in patients with recent diagnosis of SLE according to the positivity of anti-dsDNA by the Crithidia luciliae method. Lupus. 2015;24(11):1198-1203
6. Bragazzi NL, Watad A, Damiani G, Adawi M, Amital H, Shoenfeld Y. Role of anti-DNA auto-antibodies as biomarkers of response to treatment in systemic lupus erythematosus patients: hypes and hopes. Insights and implications from a comprehensive review of the literature. Expert Rev Mol Diagn. 2019;19(11):969-978
7. Zaminski D, Saxena A, Izmirly P, Buyon JP, Belmont HM. Clinical implications of discordance between anti-dsDNA antibodies by multiplex flow immunoassay and Crithidia luciliae assay in a multiethnic racial cohort of patients with SLE. Lupus Sci Med. 2023;10(2):e001012
8. Cockx M, Van Hoovels L, De Langhe E, et al. Laboratory evaluation of anti-dsDNA antibodies. Clin Chim Acta. 2022;528:34-43
Day(s) Performed
Monday through Friday
Report Available
2 to 4 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
86255
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| CRTHS | dsDNA Ab IFA Screen, IgG, S | 6457-6 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 623426 | dsDNA Ab IFA Screen, IgG, S | 6457-6 |
| 623463 | dsDNA Interpretation | 69048-7 |