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HelpTest Code CMVPV Cytomegalovirus (CMV) Molecular Detection, PCR, Varies
Ordering Guidance
For plasma specimens order CMVQN / Cytomegalovirus (CMV) DNA Detection and Quantification by Real-Time PCR, Plasma.
Necessary Information
Specimen source is required.
Specimen Required
Submit only 1 of the following specimens:
Specimen Type: Body fluid
Sources: Spinal, pleural, peritoneal, ascites, pericardial, amniotic, or ocular
Supplies: Sarstedt Aliquot Tube, 5mL (T914)
Container/Tube:
Preferred: Sterile, screwcap, 5-mL aliquot tube
Acceptable: Sterile container
Specimen Volume: 0.5 mL
Collection Instructions: Do not centrifuge.
Specimen Type: Respiratory fluid
Sources: Bronchial washing, bronchoalveolar lavage, nasopharyngeal aspirate or washing, sputum, or tracheal aspirate
Supplies: Sarstedt Aliquot Tube, 5mL
Container/Tube:
Preferred: Sterile, screwcap, 5-mL aliquot tube
Acceptable: Sterile container
Specimen Volume: 1.5 mL
Collection Instructions: Do not centrifuge.
Specimen Type: Genital swab
Sources: Cervix, vagina, urethra, anal/rectal, or other genital sources
Supplies:
-Culturette (BBL Culture Swab) (T092)
-M4-RT (T605)
Container/Tube: Multimicrobe media (M4-RT) and ESwabs
Collection Instructions: Place swab back into multimicrobe media (M4-RT, M4, or M5)
Specimen Type: Swab
Sources: Dermal, eye, nasal, saliva, or throat
Supplies:
-Culturette (BBL Culture Swab) (T092)
-M4-RT (T605)
Container/Tube: Multimicrobe media (M4-RT) and ESwabs
Collection Instructions: Place swab back into multimicrobe media (M4-RT, M4, or M5)
Specimen Type: Tissue
Sources: Brain, colon, kidney, liver, lung, etc.
Supplies: M4-RT (T605)
Container/Tube: Sterile container containing 1 mL to 2 mL of sterile saline or multimicrobe medium (M4-RT, M4, or M5)
Specimen Volume: Entire collection
Collection Instructions: Submit only fresh tissue in multimicrobe media (M4-RT) or a sterile container with 1 to 2 mL sterile saline
Specimen Type: Urine
Container/Tube: Sterile container
Specimen Volume: 1 mL
Collection Instructions: Collect a random urine specimen.
Specimen Type: Bone marrow
Container/Tube: Lavender top (EDTA)
Specimen Volume: 0.5 mL
Collection Instructions: Send bone marrow in original tube. Do not aliquot.
Useful For
Rapid qualitative detection of cytomegalovirus (CMV) DNA
This test is not intended for the monitoring of CMV disease progression.
Method Name
Real-Time Polymerase Chain Reaction (PCR)/DNA Probe Hybridization
Reporting Name
Cytomegalovirus, PCRSpecimen Type
VariesSpecimen Minimum Volume
Ocular Fluid, Spinal Fluid, or Urine: 0.3 mL
Body Fluid (pleural, peritoneal, ascites, pericardial): See Specimen Required
Respiratory Specimens: 1 mL
Tissue: 2 × 2-mm biopsy
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Refrigerated (preferred) | 7 days | |
| Frozen | 7 days |
Reference Values
Negative
Reference values apply to all ages.
Interpretation
Detection of cytomegalovirus (CMV) DNA in a specimen supports the clinical diagnosis of infection due to this virus.
Studies indicate that CMV DNA is not detected by polymerase chain reaction in cerebrospinal fluid from patients without central nervous system disease caused by this virus.
Clinical Reference
1. Binnicker MJ, Espy M. Comparison of six real-time PCR assays for the qualitative detection of cytomegalovirus in clinical specimens. J Clin Microbiol. 2013:51(11):3749-3752
2. Petito CK, Cho ES, Lemann W, Navia BA, Price RW. Neuropathy of acquired immunodeficiency syndrome (AIDS): an autopsy review. J Neuropathol Exp Neurol. 1986;45(6):635-646
3. Cinque P, Vago L, Dahl H, et al. Polymerase chain reaction on cerebrospinal fluid for diagnosis of virus-associated opportunistic diseases of the central nervous system in HIV-infected patients. AIDS. 1996;10(9):951-958
4. Broccolo F, Iulioano R, Careddu AM, et al. Detection of lymphotropic herpesvirus DNA by polymerase chain reaction in cerebrospinal fluid of AIDS patients with neurological disease. Acta Virol. 2000;44(3):137-143
5. Prosch S, Schielke E, Reip A, et al. Human cytomegalovirus (HCMV) encephalitis in an immunocompetent young person and diagnostic reliability of HCMV DNA PCR using cerebrospinal fluid of nonimmunosuppressed patients. J Clin Microbiol. 1998;36(12):3636-3640
6. Sia IG, Patel R. New strategies for prevention and therapy of cytomegalovirus infection and disease in solid-organ transplant recipients. Clin Microbiol Rev. 2000;13(1):83-121
Day(s) Performed
Monday through Sunday
Report Available
Same day/1 to 4 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
87496
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| CMVPV | Cytomegalovirus, PCR | 5000-5 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| CMVPS | Specimen Source | 31208-2 |
| 618969 | Cytomegalovirus PCR | 5000-5 |
Forms
If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.