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HelpTest Code CHIMS Chimerism Transplant Sorted Cells, Varies
Useful For
Determining the relative amounts of donor and recipient cells in a specimen in sorted cell fractions
An indicator of bone marrow transplant success
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| SORT1 | Chimerism Cell Sort 1 | No, (Bill Only) | No |
| SORT2 | Chimerism Cell Sort 2 | No, (Bill Only) | No |
Testing Algorithm
Complete chimerism analysis requires 3 specimens, under 3 separate orders, for the 3 separate tests listed below. These specimens should be submitted when collected. An interpretive report will be provided once all specimens are received.
Pre-Transplant:
-CHRGB / Chimerism-Recipient Germline (Pretransplant), Varies
-CHIDB / Chimerism-Donor, Varies
-ADONO / Additional Chimerism Donor (Bill Only), if applicable
Post-Transplant:
-CHIMU / Chimerism Transplant No Cell Sort, Varies or CHIMS / Chimerism Transplant Sorted Cells, Varies
Billing occurs with the following tests:
Pre-transplant:
-CHRGB / Chimerism-Recipient Germline (Pretransplant), Varies
-ADONO / Additional Chimerism Donor (Bill Only), if applicable
Post-Transplant:
-CHIMU / Chimerism Transplant No Cell Sort, Varies
-CHIMS / Chimerism Transplant Sorted Cells, Varies
-SORT1 / Chimerism Cell Sort 1 (Bill Only)
-SORT2 / Chimerism Cell Sort 2 (Bill Only)
For more information see Chimerism-Recipient Germline Testing Algorithm
Special Instructions
Method Name
Polymerase Chain Reaction (PCR) Amplification/Capillary Electrophoresis
Reporting Name
Chimerism Transplant Sorted CellsSpecimen Type
VariesOrdering Guidance
This test is used to determine the relative amounts of donor and recipient cells in the recipient post-bone marrow transplant specimen. Sorted cell analysis permits more detailed evaluation of chimeric status in T-cell and myeloid cell fractions. For post-bone marrow transplant evaluation of the recipient specimen, see CHIMU / Chimerism Transplant No Cell Sort, Varies
Additional Testing Requirements
Shipping Instructions
1. Specimen must arrive within 4 days of collection.
2. Collect and package specimen as close to shipping time as possible.
Necessary Information
Specimen Type is required, either as an answer to the Order Questions or on Chimerism Analysis Information (T594) if not ordering electronically. Testing will be delayed if this information is not provided.
Specimen Required
Submit only 1 of the following specimens:
Specimen Type: Blood
Container/Tube:
Preferred: Lavender top (EDTA)
Acceptable: Yellow top (ACD)
Specimen Volume: 4 mL
Collection Instructions:
1. Only 1 tube is required.
2. Invert several times to mix blood.
3. Label specimen as blood.
4. Send whole blood specimen in original tube. Do not aliquot.
Specimen Type: Bone marrow
Container/Tube:
Preferred: Lavender top (EDTA)
Acceptable: Yellow top (ACD)
Specimen Volume: 2 mL
Collection Instructions:
1. Invert several times to mix bone marrow.
2. Label specimen as bone marrow.
3. Send bone marrow specimen in original tube. Do not aliquot.
Specimen Minimum Volume
Blood: 3 mL
Bone marrow: See Specimen Required
Lesser volumes may be acceptable, depending on white cell count.
Call 800-533-1710 or 507-266-5700 with questions.
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Varies | Ambient (preferred) | 4 days | PURPLE OR PINK TOP/EDTA |
| Refrigerated | 4 days | PURPLE OR PINK TOP/EDTA |
Reference Values
An interpretive report will be provided.
Interpretation
An interpretive report will be provided, which includes whether chimerism is detected and, if detected, the approximate percentage of donor and recipient cells. Sorted cell analysis permits more detailed evaluation of chimeric status in T-cell and myeloid cell fractions, which can be helpful in clinical management.
It is most useful to observe a trend in chimerism levels. Clinically critical results should be confirmed with 1 or more subsequent specimens.
Clinical Reference
1. Antin JH, Childs R, Filipovich AH, et al: Establishment of complete and mixed donor chimerism after allogenic lymphohematopoietic transplantation: recommendations from a workshop at the 2001 Tandem Meetings of the International Bone Marrow Transplant Registry and the American Society of Blood and Bone Marrow Transplantation. Biol Blood Marrow Transplant. 2001;7(9):473-485
2. Tang X, Alatrash G, Ning J, et al: Increasing chimerism following allogeneic stem cell transplantation is associated with longer survival time. Biol Blood Marrow Transplant. 2014 Aug;20(8):1139-1144. doi: 10.1016/j.bbmt.2014.04.003
3. Ludeman MJ, Zhong C, Mulero JJ, et al: Developmental validation of GlobalFiler PCR amplification kit: a 6-dye multiplex assay designed for amplification of casework samples. Int J Legal Med. 2018 Nov;132(6):1555-1573. doi: 10.1007/s00414-018-1817-5
4. Tyler J, Kumer L, Fisher C, Casey H, Shike H: Personalized chimerism test that uses selection of short tandem repeat or quantitative PCR depending on patient's chimerism status. J Mol Diagn. 2019 May;21(3):483-490. doi: 10.1016/j.jmoldx.2019.01.007
5. Lion T, Watzinger F, Preuner S, et al: The EuroChimerism concept for a standardized approach to chimerism analysis after allogeneic stem cell transplantation. Leukemia. 2012 Aug;26(8):1821-1828. doi: 10.1038/leu.2012.66
Day(s) Performed
Monday through Friday
Report Available
4 to 8 days following receipt of Pre and Donor SpecimensPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
81268-Chimerism (engraftment) analysis, post hematopoietic stem cell transplantation specimen, includes comparison to previously performed baseline analyses; with cell selection (eg, CD3, CD33), each cell type (if appropriate)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| CHIMS | Chimerism Transplant Sorted Cells | 34574-4 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| MP025 | Specimen Type | 31208-2 |
| CHIS | Final Diagnosis | 34574-4 |
Forms
1. Chimerism Analysis Information Sheet (T594)
2. If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.