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Test Code CHIKC Chikungunya Virus, PCR, Molecular Detection, Spinal Fluid


Additional Testing Requirements


Due to the short period in which chikungunya RNA may be detected in cerebrospinal fluid (CSF), testing serum for IgM- and IgG-class antibodies to chikungunya virus is also recommended. See CHIKV / Chikungunya IgM and IgG, Antibody, Serum.



Specimen Required


Collection Container/Tube: Vial number 2

Submission Container/Tube: Sterile screw cap vial

Specimen Volume: 0.5 mL

Collection Instructions: Do not centrifuge or heat inactivate.


Useful For

Qualitative detection of chikungunya virus in cerebrospinal fluid after early symptom onset (ideally <7 days)

 

This test is not recommended for screening healthy patients.

Method Name

Real-Time Reverse Transcription Polymerase Chain Reaction (RT-PCR)/DNA Probe Hybridization

Reporting Name

Chikungunya Virus, PCR, CSF

Specimen Type

CSF

Specimen Minimum Volume

0.3 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
CSF Refrigerated (preferred) 7 days
  Frozen  7 days

Reference Values

Negative

Reference values apply to all ages.

Interpretation

A positive test result indicates the presence of chikungunya virus RNA in the specimen.

 

A negative test result with a positive internal control indicates that chikungunya virus RNA is not detectable in the specimen.

 

A negative test result with a negative internal control is considered evidence of polymerase chain reaction inhibition or reagent failure. A new specimen should be collected for testing if clinically indicated.

Clinical Reference

1. Lanciotti RS, Kosoy OL, Laven JJ, et.al. Chikungunya virus in US travelers returning from India, 2006. Emerg Infect Dis. 2007;13(5):764-767

2. Johnson BW, Russell BJ, Goodman CH. Laboratory diagnosis of chikungunya virus infections and commercial sources for diagnostic assays. J Infect Dis. 2016;214(suppl 5):S471-S474. doi:10.1093/infdis/jiw274

3. Morrison TE. Reemergence of chikungunya virus. J Virol. 2014;88(20):11644-11647

Day(s) Performed

Tuesday, Thursday

Report Available

Same day/1 to 5 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

87798

LOINC Code Information

Test ID Test Order Name Order LOINC Value
CHIKC Chikungunya Virus, PCR, CSF 81153-9

 

Result ID Test Result Name Result LOINC Value
603832 Chikungunya Virus, PCR, CSF 81153-9

Testing Algorithm

For more information see Mosquito-borne Disease Laboratory Testing

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.