Home
HelpTest Code CD40 B-Cell CD40 Expression by Flow Cytometry, Blood
Reporting Name
CD40 by Flow, QL, BUseful For
Evaluating patients for hyper-IgM type 3 (HIGM3) syndrome due to defects in CD40, typically seen in patients less than 10 years
Assessing B-cell immune competence in other clinical contexts, including autoimmunity, malignancy, and transplantation
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Specimen Type
Whole Blood EDTAOrdering Guidance
This test is not used to detect in CD40L expression (CD154), which is responsible for X-linked hyper-IgM syndrome (HIGM1); see XHIM / X-Linked Hyper IgM Syndrome, Blood.
Shipping Instructions
Testing performed Monday through Friday. Specimens not received by 4pm (CST) on Friday may be cancelled.
Collect and package specimen as close to shipping time as possible. It is recommended that specimens arrive within 24 hours of collection.
Samples arriving on the weekend and observed holidays may be canceled.
Necessary Information
Ordering healthcare professional name and phone number are required.
Specimen Required
Container/Tube: Lavender top (EDTA)
Specimen Volume: 3 mL
Collection Instructions: Send whole blood specimen in original tube. Do not aliquot.
Additional Information: For serial monitoring, it is recommended that specimens are collected at the same time of day.
Specimen Minimum Volume
1 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Whole Blood EDTA | Ambient | 72 hours | PURPLE OR PINK TOP/EDTA |
Reference Values
Present (normal)
Day(s) Performed
Monday through Friday
Test Classification
This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
88184
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| CD40 | CD40 by Flow, QL, B | 104694-5 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 89009 | CD40 by Flow, QL, B | 104694-5 |
Interpretation
This assay is qualitative; CD40 expression is reported as present (normal) or absent (abnormal). Normal B cells express surface CD40 on the majority of cells.
Hyper-IgM (HIGM3) syndrome patients typically do not express CD40 on the surface of B cells. Genotyping of CD40 is required for a definite diagnosis of HIGM3. Call 800-533-1710 for ordering assistance.
Clinical Reference
1. Bishop GA, Hostager BS. The CD40-CD154 interaction in B cell-T cell liaisons. Cytokine Growth Factor Rev. 2003;14(3-4):297-309
2. Lee WI, Torgerson TR, Schumacher MJ, et al. Molecular analysis of a large cohort of patients with hyper immunoglobulin M (IgM) syndrome. Blood. 2005;105(5):1881-1890
3. Kutukculer N, Moratto D, Aydinok Y, et al. Disseminated cryptosporidium infection in an infant with hyper-IgM syndrome caused by CD40 deficiency. J Pediatr. 2003;142(2):194-196
4.Banday AZ, Nisar R, Patra PK et al. Clinical and immunological features, genetic variants, and outcomes of patients with CD40 Deficiency J Clin Immunol. 2023;44(1):17. doi:10.1007/s10875-023-01633-1.
5. Yazdani R, Fekrvand S, Shahkarami S, et al. The hyper IgM syndromes: Epidemiology, pathogenesis, clinical manifestations, diagnosis and management. Clin Immunol. 2019;198:19-30. doi:10.1016/j.clim.2018.11.007
Report Available
3 to 4 daysMethod Name
Flow Cytometry