Test Code CAFF Caffeine, Serum
Specimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube:
Preferred: Serum gel
Acceptable: Red top
Submission Container/Tube: Plastic vial
Specimen Volume: 0.5 mL
Collection Instructions:
1. Serum gel tubes should be centrifuged within 2 hours of collection.
2. Red-top tubes should be centrifuged and the serum aliquoted into a plastic vial within 2 hours of collection.
Useful For
Monitoring caffeine therapy in neonates
Assessing caffeine toxicity in neonates
Method Name
Enzyme Multiplied Immunoassay Technique (EMIT)
Reporting Name
Caffeine, SSpecimen Type
SerumSpecimen Minimum Volume
0.25 mL
Specimen Stability Information
Specimen Type | Temperature | Time | Special Container |
---|---|---|---|
Serum | Frozen (preferred) | 28 days | |
Ambient | 72 hours | ||
Refrigerated | 72 hours |
Reference Values
Therapeutic: 8.0-20.0 mcg/mL
Critical value: ≥30.0 mcg/mL
Interpretation
Optimal pharmacologic response occurs when the serum level is in the range of 8.0 to 20.0 mcg/mL.
Toxicity in neonates and adults may be seen when the serum level is above 20.0 mcg/mL.
Clinical Reference
1. Milone MC, Shaw LM. Therapeutic drugs and their management. In: Rifai N, Chiu RWK, Young I, Burnham C-AD, Wittwer CT, eds. Tietz Textbook of Laboratory Medicine. 7th ed. Elsevier; 2023:420-453.e9
2. Brunton LL, Knollmann BC, eds. Goodman & Gilman's: The Pharmacological Basis of Therapeutics. 14th ed. McGraw-Hill Education; 2023
3. Ou CN, Frawley VL. Concurrent measurement of theophylline and caffeine in neonates by an interference-free liquid-chromatographic method. Clin Chem. 1983;29:1934-1936
Day(s) Performed
Monday through Saturday
Report Available
Same day/1 to 2 daysPerforming Laboratory
Mayo Clinic Laboratories in RochesterTest Classification
This test has been modified from the manufacturer's instructions. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
80155
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
CAFF | Caffeine, S | In Process |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
8754 | Caffeine, S | 3422-3 |
Forms
If not ordering electronically, complete, print, and send a Therapeutics Test Request (T831) with the specimen.